Koch's Postulates
The correct order of the postulates
What is C->A->D->B
Prior to staining, smears of microorganisms must be heat-fixed in order to what?
attach them firmly to the slide
Developed a set of criteria that could be used to establish a causative link between a particular microorganism and a particular disease
Koch
The primary stain
What is crystal violet
The structure labeled #1
What is a sugar bond between NAG and NAM
The microbe is found in all cases of the disease but is absent from healthy individuals
Not always true because of the presence of immune systems
The technique that is a simple way to observe microbes by placing them in a drop of water on a slide with a coverslip
Wet mount
The scientist credited for definitively disproving the theory of spontaneous generation using broth in a swan neck flask
What is Louis Pasteur
The counter stain
What is safranin
The structure labeled #2
What is a D-Alanine terminal residue of tetrapeptide (D-Ala)
You have isolated a bacterium that you believe to be the causative agent of a new disease in mice. How would you test the second postulate of Koch?
isolate the bacterium and grow a pure culture outside the mouse
Which of these numeric aperture and wave length combinations would give the best resolution
1. numeric aperture =0.8 wave length = 450nm
2. numeric aperture =0.8 wave length = 600nm
3. numeric aperture =0.8 wave length = 400nm
4.numeric aperture =0.8 wave length = 500nm
3. numeric aperture =0.8 wave length = 400nm
The nurse that realized soldiers were dying for malnutrition, poor sanitation, and microbial infection and created the polar area chart
What is Florence Nightingale
The outcome is you forgot to apply the safranin stain while performing a Gram stain
What is the Gram-negative bacteria would be colorless
The structure labeled #3
What is Diaminopimelic acid (DAP)
You have isolated a bacterium that you believe to be the causative agent of a new disease in mice. How would you test the third postulate of Koch?
inject cells from the pure culture into mice
You and a research group are performing research on aquatic microbial life in a local pond and you assignment is to take images of live microorganisms showing their organelles. What type of microscope would you use?
What is Phase-contrast microscope
The scientist credited with developing and documenting the first vaccination procedure against smallpox. (Hint: think cowpox)
What is Jenner
The type of staining that the Gram-staining procedure would be an example of
What is Differential Staining
The structure labeled #4
What is NAM (N-acetylmuramic acid)
You have isolated a bacterium that you believe to be the causative agent of a new disease in mice. How would you test the fourth postulate of Koch?
isolate a pure culture of your bacterium from the dead mouse you have previously inoculated
The term for if the objective lenses of a microscope can be changed without losing focus on the specimen
What is parfocal
Select all that apply, which of these did Louis Pasteur accomplish in the field of microbiology
1. developed swan-necked flask
2. identified the cause of anthrax as a bacterium
3. developed the first rabies vaccine and treated thousands of people
4. developed a vaccine for fowl cholera
What is all but number 2
Two things in common with Gram negative and Gram positive cell envelopes
What is Peptidoglycan and Cell Membrane
The structure labeled #6
What is Crosslink/interbridge