True/False
Plates such as the MSA, EMB, etc. can only be selective.
(False) These plates are both Selective and Differential.
Gram + plate that contains phenol red, NaCl, and Mannitol
Mannitol Salt Agar
DOUBLE JEOPARDY
What is inhibition?
Inhibition is the prevention or slowing of growth of bacteria.
What is the index case?
The first person in a population to have a disease.
Quickvue Strept Test
The three types of hemolysis are beta, gamma, and alpha.
True!
What are the two gram + positive plates we learned about?
MSA and CNA
The area that is cleared around an antibiotic disk on a Mueller Hinton plate is called the .
Zone of Inhibiton
DOUBLE JEOPARDY
How would you calculate the incidence rate?
Incidence Rate is the number of new cases of a disease / the # of people in the population at risk time 100 (percentage)
This semi-Quantitative test can determine the presence of a UTI
Urine Culture Plate
A bacteria that grow on an EMB plate is a gram - bacteria.
Partially true! EMB plate has exceptions ! Remember some species of staph and enterococcus will grow on this plate.
Why does the HE plate allow you to differentiate?
Specifiically colonies of salmonellas will produce a black ppt while colonies of shigella will have blue-green coloring with no change in the medium.
What does the abbreviation of CNA stand for?
Colisitin and Nalidixic Acid
Please come up to the board and demonstrate a urine culture plate inoculation.
1. Line straight done with volumetric loop
2. With same loop zig-zag across the straight line
DOUBLE JEOPARDY
True/FalseThe RapID ONE test is quantitative due to its use of the microcode to determine the bacteria?
FALSE! RapID one is a qualitative test. We used colors to determine positive/negative results!
DOUBLE JEOPARDY
(False) Lactose fermenters that produce acid-end products will turn the agar on a MacConkey Plate pink.
Which plate would you choose to be able tell if you have a species that vigorously ferments lactose?
EMB! This plate will change the color the of the colonies in order to determine lactose fermentation. E.coli - produces green metallic sheen
Other lactose fermenters- dark purple black colonies
What is phenol red?
A pH indicator that is used in the Mannitol Salt Agar.
On the QuickVue Strept Test, could you count the test as viable if the control window did not show the pink vertical line?
(true/false) All of the bacteria in the RapID ONE test must be Oxidase negative enterobacteria.
TRUE!
Antibiotic Resistance can develop from many different ways including natural selection, horizontal gene transfer, and random mutation.
TRUE
What would happen if you where to remove crystal violet and bile salts from the MacConkey Agar?
Gram + bacteria would grow and the plate will no longer be selective.
Which plate should I use to differentiate enterobacteria based on their ability to ferment lactose?
What must be present on the QuickVue strept test in order to count it as a true positive?
Test must have vertical pink line in the test result window AND in the control window. Along with the blue in the test complete window.
The Kirby Bauer Disk Diffusion Assay is method used to determine the antibiotic susceptibility of bacteria on a Mueller-Hinton plate. Antibiotic disks are placed on a lawn of bacteria and zones of inhibition are measured and compared against standards to determine if the bacteria is resistant or susceptible. It is used in clinical laboratories to help determine what would be the best antibiotic to use against a bacteria.