The biochemical test that distinguishes Staphylococcus from Streptococcus is...
catalase
If a patient is positive for Influenza A, what test is reflexed?
H5N1
What flag do you need to put when processing a pediatric blood bottle?
no anaerobic bottle
What makes mycobacteria different than bacteria?
thick waxy cell wall rich with mycolic acids
This gram stain result is diagnostic for Neisseria gonorrhoeae in males...
intracellular gram-negative diplococci
What pathogens are part of the VP test?
Bacterial vaginosis, Candida spp., Trichomonas vaginalis
When working up a blood culture, what plates do you need if you see GPC and GNB on gram stain?
BAP, CHOC, BBA, MAC, CNA
Mycobacterium gordonae
Considering the following results, what stage of infection does this patient have?
Positive HBsAg
Negative Anti-HBc IgM
Positive Anti HBc
Chronic HepB infection
What are the 3 steps of PCR?
Denaturation, annealing, and extension
What plates are added and why for tissue cultures with sources below the knees and hands?
30 degree chocolate and 7H11 for Mycobacterium marinum
zoophilic fungus
Microsporum canis
What organism grows well on chocolate, causes genital ulcer disease, and requires X factor?
Haemophilus ducreyi
Which of the following pathogens would use reverse transcriptase PCR?
A: Herpes simplex virus
B: Coronavirus
C: CMV
Coronavirus since it is an RNA virus
What plate should be added for bile/gallbladder source?
Hektoen
This type of Aspergillus species is dark green and can grow up to 50 degrees celsius
Aspergillus fumigatis
A patient who recently ate raw fish is experiencing stomach pain and diarrhea. A gram stain shows curved gram-negative rods and is sucrose negative. What is it?
Vibrio parahaemolyticus
What methodology does the biofire use?
Melt curve analysis
You have a sputum culture with numerous epithelial cells, very numerous PMNs, and predominant GNB. What would you do?
Not reject due to the PMNs that are 10x greater than the epis and a predominant organism
A patient with a lung infection recently visited the Midwest and was hiking in an area with lots of bird/bat droppings. What fungus is this caused by?
Histoplasma capsulatum