An organism is placed onto a slide with a loop. A drop of a reagent is placed on the organism. There is no bubbling seen. This is a negative reaction to the _______ test.
What is the catalase test?
100
In this type of microscopy the condenser directs light at oblique angles away from the objective lens in a manner that allows only objects in the field of view to redirect or scatter light into the objective lens.
5 – 7 mL of media which has solidified at an angled position
What is a slant?
100
This organism is both urease positive as well as indole positive.
What is proteus?
200
This test produces a dark blue precipitate when a microorganism is positive.
What is the lipase test?
200
What is the equation for resolution?
What is d = wavelength / 2NA
200
Clostridium is part of a/can be tested for in a..
What is a spore stain?
200
5 – 7 mL of media which has solidified in an upright position.
What is a deep?
200
Metallic green sheen on EMB
What is e. coli?
300
This tests for a 2,3 Butanediol fermentor. Enterobacter is positive for this test.
What is the Vogues-Proskauer test?
300
How can you improve d?
What is by decreasing wavelength or increasing NA?
300
Name all 4 liquids in a gram stain.
CV, iodine, ethanol, safranin.
300
15 – 20 mL of liquid agar used in a plate
What is a pour?
300
This organism is positive for the Voges Proskauer test
What is e. aerogenes?
400
What does the Simmons Citrate test test for?
What is the ability for a microorganism to use citrate as its sole carbon source?
400
This part adjusts the diameter of the cone of light so that it just fills the objective lens
What is the iris diaphragm?
400
What is the purpose of steaming microorganisms?
To force the dye into the organism because they are heat and liquid resistant.
400
DAILY DOUBLE!
Name 2 differences between natural and synthetic media.
-Natural : components unknown, raw materials.
synthetic: Components known, can be specific with certain types of media.
400
This species of organism does not react with the catalase test.
What is strep?
500
MSA plates have a ______% salt concentration while Staphylococcus 110 Medium has a _____% salt concentration.
What is 7.5% and 7.5%? **Trick question. :)
500
Which one of these is false?
As you close down the diaphragm:
1. The light intensity decreases
2. Contrast improves
3. Depth of field decreases
4. Limit Resolution
What is Choice 3?
500
Water. (The title of this is STAINS and SHAPES. What does it have to do with STAINS?)
What is a decolorizer in spore stains?
500
This type of media chooses between different types of microorganisms based on appearance of growth or color changes