Staining
A stain using one dye to visualize cell anatomy under a microscope
What is a simple stain?
Use soap and water to do this at the beginning and end of lab
What is wash your hands?
This hardy bacteria contains mycolic acid on its cell wall and causes tuberculosis
What is Mycobacterium tuberculosis?
This liquid media does not contain agar
What is broth?
You applied hydrogen peroxide to bacteria in this assay
What is the catalase assay?
This differential stain turns cells pink or purple based on the thickness of the peptidoglycan layer in their cell wall
What is Gram Staining?
Spray this on your bench top before and after your experiments with bacteria
What is disinfectant?
This endospore forming Gram negative bacteria is the most common cause of HAIs today
What is Clostridioides difficile (C. diff)?
This agar was used to screen for hemolytic bacteria
What is blood agar?
A way to verify that the reagents and procedures work correctly
What are positive and negative controls?
This technique stains the slide, not the cells
What is a negative or capsule stain
This part of a glass culture tube is flamed before and after transfer
What is the lip or mouth of the tube?
This endospore forming bacteria has been used in bioterrorism in the US
What is Bacillus anthracis?
This media abbreviated TSA is an "everyday agar", not differential or selective.
What is tryptic soy agar?
If a Durham tube in the phenol red assay contains a bubble, the bacteria have performed this type of metabolism
What is fermentation with gas production?
This is the last step in making a smear before applying stains
What is heat fixing?
Flaming the loop kills all microbes through this oxidative process
What is incineration?
This bacteria forms a capsule but does not cause the flu as its name suggests.
What is Haemophilus influenzae?
This dark green agar selects for Gram-negative enteric bacteria like Salmonella and Shigella
What is Hektoen agar?
Do this to a semi soft agar tube to test for motility
What is stab?
"Come In And Stain!" is the mnemonic used to remember these steps in Gram staining
What is crystal violet, iodine, alcohol, and safranin
Cover your Petri dish with a tilted lid during inoculation to avoid this
What is contamination?
This motile Gram negative bacteria was indole positive in the TSIA assay
What is Proteus vulgaris?
This red agar selected for enteric bacteria and can differentiate between bacteria that metabolize lactose
What is MacConkey Agar?
Indole turns pink with this reagent
What is Kovac's reagent?