Microscopy
Staining Basics
Cell morphology
Gram stain
Catalase Test
100

The name of the objective lens with the yellow ring (10x).

What is Low power?

100

A differential staining technique will use this many stains/dyes.

What is 2?

100

This term is used to describe spherical-shaped bacterial cells.

What is coccus?

100

The primary stain used in the gram stain.

What is Crystal Violet

100

In the catalase test, this is used as the chemical reagent used to detect the presence of catalase.

What is Hydrogen peroxide?

200

The microscopes used in this class have a double magnifying lens system.  Microscopes that have this lens system are called ________ .

What are Compound microscopes

200

This is the type of stain used in the negative staining technique.

What is an Acidic stain like carbol fusion

200

This term is to describe rod-shaped bacterial cells.

What is bacillus?

200

This major structure of the bacterial cells differs between gram-positive cells from gram-negative cells.

What is the Cell wall?

200

If a bacterial specimen produces catalase, you would be able to see this as a result.

What are Bubbles?

300

This is the total magnifying power of your specimen when using the oil immersion lens.

What is 1000X?

300

When completing a simple stain, this is the type of stain you would use.

What is a basic stain, like crystal violet or methalyne blue?

300

This term is used to describe the cellular arrangement that looks like a cluster of cells.

What is staphylo?

300

The color of gram-negative cells at the end of the gram-staining technique.

What is Red/pink?

300

These are the 2 products from the breakdown of hydrogen peroxide by catalase.

What are water and oxygen?

400

When using the microscope, it is always important to adjust your light accordingly.  As you increase in magnifying power, should you increase or decrease your light?

What is increase?

400

When making a traditional bacterial smear you are required to heat fix the smear.  This is because heat fixation accomplishes these 2 things.

What is kills the microbes and fixes/adheres the microbes to the slide?

400

This term is used to describe a cellular arrangement that looks like a chain of cells.

What is strepto?

400

If you failed to apply the counterstain but correctly completed all other steps, gram-positive cells would be this color.

What is Purple?

400

A non-catalase-producing microbe would have this aerotolerance.

What is an obligate/strict anaerobe?

500

When using the oil immersion lens, if you are unable to fine focus for visualization, you should adjust to this lower magnifying objective lens so can refocus.

What is the Scanning objective lens (4x)

500

The result of a negative stain shows a black background with translucent cells because this charge in the dye is being repelled by the bacteria.

What is a negative charge.

500

What is the visible structural difference between spirochete and spirillum 

What is the number of helical turns?

500

If you over-decolorized your smear during the gram stain technique but did all other steps correctly, this would be the color of your gram-positive cells.

What is Red/pink?

500

This type of microbe can cause hemolysis. 

What are particular species of parasites, bacteria, and viruses