Microscopy
This adjustment is used first to bring a specimen into general focus using the lowest objective
What is coarse adjustment?
This color indicates Gram-negative bacteria after staining.
What is Pink/red?
A clear area forms around an antibiotic disk where bacteria fail to grow
What is a zone of inhibition?
This type of media allows only certain bacteria to grow.
What is selective media?
This color change shows that bacteria fermented a sugar
What is red to yellow?
This structure focuses light directly onto the specimen
What is the condenser?
This step fixes cells to the slide using heat
What is Heat fixation?
This laboratory method determines bacterial susceptibility to antibiotics by measuring clear zones around disks.
What is the Kirby-Bauer method?
This type of media distinguishes bacteria based on metabolic activity
What is Differential media?
Growth spreading away from the stab line in this test shows that the bacteria can move
What is a positive motility result in a SIM test?
This component controls light intensity and improves contrast by blocking scattered light.
What is the Iris diaphragm?
This is the primary stain used in Gram staining
What is crystal violet?
A bacteria shows moderate inhibition around an antibiotic disk, suggesting treatment may work under certain conditions or doses.
What is intermediate susceptibility?
This color change indicates carbohydrate fermentation in phenol red broth
What is Red to yellow?
This enzyme allows bacteria to break down gelatin
What is gelatinase?
This adjustment is used after coarse focus for sharp, detailed clarity
What is fine adjustment?
This error causes all cells to appear pink
Over-decolorization
In disk diffusion testing, these clear areas are measured and compared to reference values to determine resistance.
What are zones of inhibition (measured in millimeters)?
This enzyme breaks down hydrogen peroxide in a catalase test
What is catalase?
This reaction shows up as bubbles when hydrogen peroxide is added
What is a positive catalase test?
This term describes the ability to distinguish two nearby objects as separate
What is resolution?
This chemical acts as a mordant in Gram staining
What is Iodine?
This term describes substances that stop bacterial growth without killing the organisms.
What is bacteriostatic?
This appearance on EMB agar indicates strong lactose fermentation.
What is a Metallic green sheen?
A quick dark color change on test paper shows this enzyme is present
What is oxidase (cytochrome c oxidase)?