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Stains
Media
Eukaryotic Microbes
Unknown Tests
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100
Gram positive organisms are this color at the end of a gram stain.
What is the color purple?
100
An autoclave is used to kill these in order to sterilize media.
What is microorganisms and endospores?
100
This eukaryotic organism contains a pseudopod and is able to undergo phagocytosis.
What is an Amoeba?
100
The positive result for this test is the formation of bubbles after H2O2 has been added.
What is the catalase test?
100
The ocular lens has this magnification.
What is 10X?
200
This stain uses a basic stain and an acidic stain but the organism is not stained in the final image.
What is a capsule stain?
200
This type of media allows the growth for only specific microbes.
What is selective media?
200
This is an example of green algae you saw in class.
What is Spirogyra?
200
This media contains 7.5% NaCl and will select for bacteria that can withstand the high salt concentration.
What is Mannitol Salt Agar?
200
This test determines if the bacterium can hydrolyze the amino acid tryptophan.
What is Indole Test?
300
An iodine mordant is used to complex with this primary stain.
What is crystal violet?
300
This media is used to promote the formation of endospores.
What is nutrient sporulation media?
300
This organism has an oral groove, moves by cilia and divides by transverse fission.
What is paramecium?
300
Stabbing solid thioglycolate media is used to test this characteristic of microorganisms.
What is aerotolerance?
300
This organism undergoes Beta Hemolysis when plated on blood agar.
What is S. aureus?
400
This is an example of a basic stain.
What is methylene blue, crystal violet or safranin?
400
This media selects against gram positive organisms.
What is MacConkey Agar?
400
This organism enables us to enjoy beer and bread.
What is Saccharomyces cerevisiae?
400
Normal agar is 1.5%. The agar used to test for motility is this percentage agar.
What is 0.4%?
400
This is the pressure and temperature used in the autoclave.
What is 15 psi and 121 degrees celsius?
500
Congo red, Eosin and nigrosin are examples of this stain.
What is an acidic stain?
500
This is an example of a complex media.
What is TSA or TSB?
500
This type of organism includes Amoeba, Paramecium, Spirogyra, Volvox and Euglena.
What is protozoa?
500
This test is used to detect bacteria that undergo mixed acid fermentation.
What is the methyl red test?
500
Anything less than this percentage is considered a different species when comparing 16S rRNA.
What is 97-98%?