Gram stain
Step 1
An air-dried, heat-fixed smear is thoroughly covered with the purple dye.
Test used to determine the chemical composition and thickness of the cell wall
What is a gram stain?
Round-shaped
What is coccus?
Require a much lower level of oxygen about 5%
What is a microaerophiles?
The ability to survive, grow, develop, and potentially reproduce
What is viability?
Step 2
After 30 seconds the stain is washed off with water and the smear is covered with iodine.
Laboratory test used to determine to which bacteriophages a bacterium is susceptible to lysing, aids in determining the origin of a disease.
What is phage typing?
Rod-shaped
What is bacillus?
Require oxygen that is similar to ambient room air about 20-21%
What are faculative aerobes?
To classify microbes we determine their nutritional requirements or sources of energy and _________.
What is carbon?
Step 3
After 30 seconds the iodine is washed off with water. Both gram-positive and gram-negative bacteria appear purple at this point.
Test used to identify the S. aureus species
What is a coagluase test?
Ridged, thickened spirals
What is spirillum?
Can survive with or without oxygen.
What are facultative anaerobes?
Microscope used to see 3 dimensional views of the smallest microbes, viruses, and cellular structures
What is a scanning electron microscope?
Step 4
The slide is now washed with the decolorizing agent called ethanol. The ethanol removes the purple stain from some bacteria and not from others.
Used to determine the motility of a specimen
What is a hanging drop test?
Flexible, thin spirals
What is spirochetes?
Grow without oxygen but not killed by it
What is aerotolerant anaerobes?
The process of removing water, completely drying up; method of preserving by dehydration
What is desiccation?
Step 5
The alcohol is rinsed off and the slide is stained with a basic red dye. The stain is left on the slide for 1minute, rinsed with water, blotted dry and examined under immersion oil.
Type of testing has been used to determine what is known as DNA fingerprinting in root cause analyses of healthcare-associated infections?
What is the Nucleic Acid-Base composition?
Comma-shaped or C-shaped
What is vibrios?
Killed by oxygen
What are obligate anarobes?
Invaginations (folding inward to form a pouch-like cavity) within the cytoplasmic membrane is known as
What are mesosomes?