Mycobacteria
Virology
Unculturables
Anaerobes
Cell Wall Deficient Bacteria
100

What concentration of sodium hydroxide (NaOH) is used to prepare a working decontamination solution for the processing of not normally sterile specimens for mycobacteria?

What is 2-4%?
100

Which virus is the most common etiological agent of viral respiratory disease in infants and children?

What is Respiratory Syncytial Virus (RSV)?
100

Rickettsia rickettsii and Ehrlichia chaffeensis are both transmitted by which arthropod vector?

What are Ticks?

100

A gram positive spore forming bacillus growing on anaerobic blood agar (CDC) produces a double zone of beta hemolysis and is positive for lecithinase. What is the presumptive identification?

What is Clostridium perfringens?

100

Primary atypical pneumonia is caused by....

What is Mycoplasma pneumoniae?

200

Mycobacteria stained by the Ziehl-Neelsen or Kinyoun methods with methylene blue counterstain are seen microscopically as...

What are Bright red rods against a blue background?

200

Which virus belonging to Reoviridae group causes gastroenteritis in infants and young children but an asymptomatic infection in adults?

What is ROTAVIRUS?
200

Which specimen would be best for isolation of Chlamydia trachomatis from a female patient with a genital infection?

What is an Endocervical swab?

200

Which Clostridium species caused antibiotic associated diarrhea?

What is CLOSTRIDIUM DIFFICILE?
200

Which organism typically produces “fried egg” colonies on agar within 1-5 days of culture from a genital specimen?

What is Mycoplasma hominis?

300

Acid-fast staining of a smear prepared from a first morning expectorated sputum showed slender, slightly curved, beaded, red mycobacterial rods. Growth on Middlebrook 7H10 slants produced buff-colored colonies with a serpentine pattern after 14 days at 37C. Niacin and nitrate reduction tests were positive. What is the most probable presumptive identification?

What is Mycobacterium tuberculosis?

300

Which is the first antibody to appear in recurrent or acute Hepatitis B infection?

What is IgM anti-HBc?

300

Which organism is the causative agent of Q fever?

What is C. burnetii?

300

A necrotic ulcer has grown on the foot of a patient with poorly controlled Diabetes mellitus. Cultures of the wound are sent to the Microbiology lab. The resulting colonies are catalase positive, emit a foul odor, and turn the BBE plate black. What antibiotics, commonly used in anaerobic media, will this organism be resistant to?

What are KANAMYCIN AND VANCOMYCIN?
300
What are the preferred methods of testing for Mycoplasma and Ureaplasma?
What are SEROLOGY AND MOLECULAR METHODS?
400
A non-tuberculous mycobacterium that produces pigment when exposed to light is called....
What is a PHOTOCHROMOGEN?
400

A 19 year old patient visited the college health center with complaints of extreme fatigue, fever, sore throat, and swollen lymph nodes. Her serological work up yielded the following results:

Anti-VCA (IgM): positive

Anti-VCA (IgG): positive

Anti-EA-D: positive

Anti-EA-R: negative

Anti-EBNA: negative


Based on these results, the most likely cause of the patient’s illness is:


What is Acute infectious mononucleosis?

400

Which organism is the causative agent of leprosy?

What is M. leprae?

400
What 4 types of anaerobic media should be included in a traditional anaerobic culture?
What are ANAEROBIC BLOOD AGAR (CDC), PEA, LKV, AND BBE?
400

The manganous chloride-urea test is used for the identification of which organism?

What is Ureaplasma urealyticum?

500
Which NTM is a scotochromogen when incubated at 37C and a photochromogen when incubated at 25C?

What is M. szulgai?

500

According to the updated algorithm for HIV testing from the CDC (2014), a positive result on an HIV-1/2 antigen/antibody combination immunoassay should be followed by:

What is HIV-1/HIV-2 antibody differentiation immunoassay?

500

The principle of which test is based on cross reactivity between Rickettsia and some Proteus species?


What is the WEIL-FELIX TEST?
500

Which gram negative anaerobic bacilli produce brick red fluorescence under ultraviolet light?

What is Porphyromonas?

500
Which autoantibody is associated with previous Mycoplasma pneumoniae infection?
What is Anti-I?