Respiration
How many ATP are created in Aerobic respiration?
38
What does EMB and MAC stand for?
What are their properties?
Eosin Methlyene Blue medium
MacConkey agar
BONUS: what bacteria is commonly used on EMB
e. coli, salmonella, shigella
properties: selective for gram -, selective against gram +, differential for lactose fermentation
What was initially thought about DNA?
that DNA was too simple to carry genetic information
name the two purposes of a Durham tube
collects CO2
detects fermentation with acid and gas
Flesh-eating disease
Necrosis
How much ATP is created during fermentation?
2
give the definition of fastidious
have complex nutritional requirements
when was nuclein discovered?
mid 1800's
BONUS: what is nuclein?
can viruses be cultured on artificial media?
no
reproduction of yeast
budding
What takes place during the electron transport chain?
what is generated during the electron transport?
oxidization of NADH and FADH
bonus!!
What is NADH and FADH worth?
the proton gradient is generated
what is blood agar differential for?
staph & strep
they both have ribosomes and DNA
what is thioglycolate medium?
it is a reducing agent that decreases the amount of O2
Give the genus names of two obligate anaerobes
clostridium bacteriodes
Examples of fermenters (4)
yeasts - saccharomyers
clostridium
propionic acid bacteria
facultative anerobes, e. coli
what is the definition of differential and selective?
selective - prevents growth of some bacterial types while allowing others to grow
differential - has a certain type of media that allows certain bacteria to grow
BONUS: two examples talked about in class
when was the shape of DNA discovered?
1910
BONUS: who was it discovered by?
rosalind whatever
what is RNA polymerase
lactose and mannitol
BONUS: what is the other carbohydrate fermentation?
glucose
"grooming" of pyruvate for oxidation in Kreb's cycle occurs in what stage?
transition phase
BONUS: how does pyruvate get broken down?
by co enzyme A it gets turned into Acetyl CoA
list the 3 biochemical tests discussed in class, what it tests for, what bacteria is used, and which ones are positive and negative for the test.
Oxidase - neisseria + and pseudomonas +
Catalase - staph + strep - uses hydrogen peroxide
Amylase - bacillus species + tests for starch
name the 5 nucleotides
Guanine
Cytosine
Thymine
Uracil
Adenine
BONUS if they same the names and not just writing down the letters.
This media is enriched and differential
blood agar (BAP)
BONUS: what is blood agar composed of?
sheeps blood find in notes.
it is a medium that has a defined amount/number of ingredients