Selective for: gram-negative bacteria
growth of gram-positive bacteria (e.g.: Staphylococcus aureus) is inhibited by the crystal violet dye and bile salts in the media –
What is Mannitol Salt Plate
100
Partial destruction of RBC’s with some loss of hemoglobin.
What is Alpha Hemolysis?
100
Bacteria that require oxygen to survive.
What are aerobes?
100
Gloves, gown, goggles, etc.
What is PPE?
200
Grows only certain types of bacteria (gram-negative or gram-positive types) or fungi
What is selective media?
200
This agar is used to confirm that you have S. aureus or S. epi.
What is Mannitol Salt Agar?
200
Clear, transparent zone around the colonies—may also have an extra halo called DOUBLE ZONE
What is Beta Hemalysis
200
Immediately after inoculation
bacteria absorbs nutrition and increases in size
What is lag phase?
200
Aseptic technique
What is always observed when transferring or working with infectious agents or specimens
/
300
Contains elements that differentiate between certain types of bacteria (lactose fermenters or hydrogen sulfide producers)
What is differential media?
300
MSA
What is Mannitol Salt Agar?
300
No hemolysis or destruction. There will be a color where the bacteria grows on the red agar but there is no cell destruction.
What is Gamma Hemalysis?
300
Bacteria killed or whose growth is inhibited in the presence of oxygen
What are anerobes?
300
Objective used to differentiate stained bacteria.
What is 100X—(oil immersion)?
400
A tube agar that has been allowed to gel at an angle
What is slant media?
400
General-use medium specially formulated to give standardized results during antibiotic sensitivity testing
What is Mueller Hinton?
400
Bacteria can/will display either Beta or Gamma hemolysis.
What is Gram Negative?
400
(best stage for testing)
Growth stabilizes due to decreased nutrients
What is stationary phase?
400
Most veterinary cultures are grown at
37° C including reptile and amphibian cultures.
What is the temperature that is especially critical when performing standardized tests as the
Kirby-Bauer sensitivity?
500
A tube agar that has been allowed to gel at an angle.
What is a slant agar?
500
Differentiates colonies on the basis of lactose fermentation
What is Salmonella-Shigella agar?
500
Bacteria may display Beta (double zone) or Gamma hemolysis. Usually NEVER Alpha.
What is Gram positive?
500
Organisms that can survive in the absence of oxygen but with limited growth
What are Facultative anaerobes?
500
Identification of these organisms, including bacteria, fungi, parasites, and viruses that cause clinical illness.