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DNA Replication
Organization of DNA
Viral Replication
Genes
Quorum Sensing
100
What does the Origin Binding Protein do in E. coli?
Initiates replication for at OriC
100
How many oriC's do eukaryotes have?
trick question - OriC is found specifically in E. coli. Eukaryotes do have MULTIPLE origins of replication
100
Which phase can the phage integrate with the host chromosome
lysogenic
100
the F-plasmid is an example of what? What does it do?
Episome - can integrate with host genome
100
What does the QS system of V. fisheri do?
bioluminescence
200
What polymerase is responsible for proofreading?
DNA pol III
200
How are eukaryotic chromosomes condensed (which two steps) and by what proteins?
DNA wraps around histones to form nucleosomes. Nucleosomes further condense into chromatin.
200
When Cro is high, which promoter is inhibited initially?
PL
200
What is an Hfr?
a bacterium with a conjugative plasmid (for example, the F-factor) integrated into its chromosomal DNA.
200
What type of QS system does S. aureus have? What type of signaling molecules does it use?
a two-component system; oligopeptide autoinducer
300
What enzyme is responsible for initiating replication on the lagging strand
Primase
300
How are supercoils formed and held together?
DNA gyrase/topo2 introduces supercoils and proteins hold them together
300
What does PR' initiate?
viral capsid proteins and lytic genes
300
What does sigma factor 70 do and where?
initiates transcription at the -10 and -35 regions in E. coli
300
What kind of communication does LuxS mediate?
inter-species
400
How will a deleterious mutation in the polA gene affect replication?
failure to remove RNA primers
400
The trp Operon is regulated by (2 terms)
repression and attenuation
400
In the late lytic pathway, name the 5 genes that the N protein promotes
cro, cII, O, P, Q
400
In eukaryotes, what is the site for initiation of transcription and where is it located?
TATA box -25
400
Name the receptor that AIPs bind to and the intracellular factor that induces transcription
ArgC (kinsae); ArgA (response regulator)
500
What enzyme is responsible for preventing concatamer accumulation in rolling circle replication?
Topo IV (4)
500
When the lac operon is initiated, what happens to the 1) CAP binding site 2)glucose levels 3) cAMP levels
1) CAP/cAMP binds to CAP BS 2) glucose would be low 3) cAMP high
500
Explain the steps that a SSDNA+ virus would take to synthesize mRNA (3 steps)
1)use DNA pol to generate complementary strand DNA- 2)Create a dsDNA intermediate 3) Uses RNApol to transcribe negative strand template
500
How does an F-plasmid integrate into the host genome?
via IS elements (insertion sequence). It then creates an Hfr - high frequency of recombination
500
In chemotaxis, name the initial receptor and response of the cell
CheA (kinase); run and tumble response