OIT MLS Chem415 Final Review Part 1

Analytical Methods

100

T/F stray light refers to any wavelengths outside the band transmitted by the monochromator.

True

200

The area between the upper limit of quantitation and the lower limit of quantitation is know as: 

The linear range

300

This is the color of visible light seen at 400 nm

Violet

400

This type of analytical method measures the amount of light that can pass through a sample at 180 degree angle from the incident light.

Turbidimetry

500

Calculate the % transmittance through a tube containing 5 layers of solution with an absorbance of 20%.

33%

Enzymes

100

This test can be used to monitor alcoholics with liver toxicity.

GGT

200

This enzyme has a short half-life and is found in the heart, liver, skeletal muscle, and kidney.

AST

300

Which test has greater specificity to liver damage.

ALT

400

What is the order that would be expected to be seen in LD isoenzymes in patient who has had a heart attack.

LD1>LD2>LD3>LD4>LD5

500

In this state, not all enzyme sites are saturated with substrate and substrate concentration is the rate-limiting step.

First-order kinetics

Amino Acids and Proteins

100

The most common secondary structure of a protein is:

Alpha helix

200

Proteins with 10-40% carbohydrates attached are:

Glycoproteins

300

This protein's primary function is to bind free hemoglobin.

Haptoglobin

400

Which protein has a function to maintain oncotic pressure?

Albumin

500

What protein is a negative acute phase reactant?

Transferrin

Non-Protein Nitrogen Compounds

100

What test serves as the glomerular filtration rate for clinicians?

Creatinine

200

Plasma creatinine levels are not affected by: 

Dietary intake of protein

300

Individuals with gout often are seen to have elevated levels of this: 

Uric acid

400

What is the pathological condition in which the proximal tubules in the kidneys do not reabsorb glucose, amino acids, uric acids, and bicarbonates resulting in their loss to the urine concentrate.

Fanconi Syndrome

500

The most frequently used analytic method for creatinine testing is: 

Jaffe reaction

Random

100

This test may be used to assess nutritional status

Prealbumin

200

This is a disease which can occur as a result of severe protein and calorie deficiency

Marasmus

300

What is an energy source utilized by muscles?

Creatine phosphate

400

In the stomach _____ break down proteins into peptides.

Pepsin

500

Given the following information, calculate the creatinine clearance:

Urine creat: 87 mg/dL  Plasma creat: 3.2 mg/dL

Urine vol/24 hrs: 810 mL   Patient BSA: 1.92

13.7