Neisseria - general characteristics
Expected gram stain for Neisseria
If N. meningitidis is suspected in a CSF, this is the temperature the specimen should be held at until it can be plated
What is room temperature (20-25 degrees Celsius)?
Expected gram stain for Haemophilus
What is pleomorphic gram-negative bacilli?
Agent of whooping cough
What is Bordetella pertussis?
What is pleomorphic, club shaped, palisading, non-branching Gram-positive bacilli, often called Chinese letters?
GPB associated with anthrax
What is Bacillus anthracis?
Unacceptable: what is stool, surface skin swab, sputum, urine? Acceptable: what is aspirate, body fluid, tissue?
Clostridium species associated with pseudomembranous colitis
What is Clostridium difficile?
Expected oxidase reaction for Neisseria
What is positive?
In this gender, it is acceptable to diagnose a Neisseria gonorrhoeae infection from a gram stain that shows many WBCs and intracellular GNDC
What is a male?
The factors required for the growth of Haemophilus sp
What are X and V factors?
A common source of Legionella species
What is water systems?
Gram stain reaction for Bacillus species
GPB associated with diphtheria
A characteristic that if noticed in a clinical specimen, is presumptive for the presence of anaerobes
What is a foul odor?
Clostridium species associated with tetanus
What is Clostridium tetani?
Atmospheric growth requirement for Neisseria
What is increased CO2 (capnophilic)?
Expected CTA sugar results for Neisseria gonorrhoeae (glucose, maltose, lactose, sucrose)
What is G+, M-, L-, S-?
The media that contains the factors required for the growth of most Haemophilus sp
What is chocolate agar?
Most common species of Pasteurella isolated in the lab
What is Pasteurella multocida?
Gram-positive bacilli that is often gram-variable and associated with clue cells
What is Gardnerella vaginalis?
GPB associated with bacterial vaginosis
What is Gardnerella vaginalis?
Anaerobic organism that demonstrates a double zone of beta hemolysis
What is Clostridium perfringens?
Clostridium species associated with botulism
What is Clostridium botulinum?
Expected CTA sugar results for Neisseria meningitidis (glucose, maltose, lactose, sucrose)
What is G+, M+, L-, S-?
Organism that causes tularemia and can be contracted from rabbits that harbor it
What is Francisella tularensis?
GPB that exhibits umbrella motility pattern in motility medium
What is Listeria monocytogenes?
GPB associated with food poisoning (think fried rice and this IS serious!)
What is Bacillus cereus?
Cutibacterium acnes is most often associated with this
What is blood culture contamination?
Clostridium species associated with gangrene
What is Clostridium perfringens?
Organism from the HACEK group that is associated with human bite wounds
What is Eikenella corrodens?
GPB associated with meningitis, infection in neonates, pregnant women and outbreaks of food contamination
What is Listeria monocytogenes?
Anaerobe (genus and species) that grows brown to black colonies on the BBE agar
Anaerobe (genus only) that is gram-negative, non-spore forming and is shaped as long, thin, tapered, "fusiform" bacilli
What is Fusobacterium species?
Anaerobe (genus only) that is sensitive to vancomycin and fluoresces brick red under UV light
What is Porphyromonas species?