Molecular Lab Basics
Contamination in molecular testing is due to this product.
What is amplified DNA or amplicons?
The instrument used for PCR amplification.
What is a thermocycler?
A Southern blot detects this.
What is DNA?
The restricted movement of samples and personnel between molecular areas.
What is unidirectional workflow?
Name for a RNA dependent DNA polymerase.
What is reverse transcriptase?
The most common and widely used amplification method.
What is PCR?
Used to monitor for contamination in every test run.
What is a negative control?
Cycle number at which a sample rises above the background.
What is the crossing point or threshold cycle?
Molecular area where specimens are received, stored and processed for testing.
What is the sample preparation area or room?
The steps in a PCR cycle.
What is denaturation, annealing and elongation or extension?
The genetic makeup of an organism.
What is genotype?
Amplified PCR products analyzed and visualized in a plot based on their hybridization formation.
What is melting analysis?