Nucleic Acid
This required for protein synthesis.
What is rRNA?
The instrument used for PCR.
What is a thermocycler?
This method detects DNA after restriction endonuclease digestion.
What is Southern blot?
Restricted movement of samples and personnel in the molecular laboratory.
What is unidirectional workflow?
A protective shell made of protein.
What is a capsid?
The nucleotides found in DNA.
What is adenine, cytosine, guanine and thymine?
The cycle number that a sample reaches a level above the background signal.
What is the cycle threshold (Ct)? also known as the crossing point
The The spectrophotometric absorbance for DNA/RNA is ______.
What is 260? protein is 280
The three areas for sample preparation and testing in the molecular laboratory.
What is reagent, sample and amplification (testing)?
The enzyme responsible for creating DNA from an RNA template.
What is RNA-dependent DNA polymerase? reverse transcriptase
The type of bond that holds DNA (dsDNA) strands together.
What is a hydrogen bond?
The steps in each PCR cycle.
What is denaturation, annealing and extension or elongation?
Name two hybridization techniques and what they detect.
What is Western blot (protein), Northern blot (RNA), ?
The purpose of molecular testing in the clinical laboratory.
What is detection of infections, polymorphisms, inherited diseases and to classify neoplasms?
Viral replication steps.
What is attachment, entry, uncoating, replication/translation, assembly and release? maturation for some viruses
This nucleic acid can be monocistronic or polycistronic.
What is messanger RNA (mRNA)?
This analysis is performed on a PCR product to verify it is correct.
What is a melt-curve analysis? based on the melting temperature of the product (Tm)
The three types of nucleic amplifications.
What is target, probe and signal?
The term used for amplified pieces of nucleic acid.
What is an amplicon?
Stages of viral disease progression.
What is incubation, prodromal stage, illness and resolution? convalescence stage when symptoms subside
The largest type of RNA found in cells and is involved in translation.
What is ribosomal RNA (rRNA)?
The three distinct phases of PCR amplification curve.
What is lag phase, exponential phase and plateau phase?
This nucleic acid amplification method occurs at a single temperature.
What is isothermal? nucleic acid sequence-based amplification (NASBA) and transcription-mediated amplification (TMA)
Replication, transcription and translation are known as
What is the Central Dogma?
The ability for a virus to bind neurons and muscle cells but not endothelial cells is known as the _________ of a virus.
What is tropism?