Trash talk
True or False: Ice packs, dessicants, and dry growth media can be safely autoclaved.
What is false.
1. Ice packs- disinfect exterior before disposing in regular trash
2. Dessicants can cause autoclave to explode.
If no infectious materials dispose through CHATS.
If potentially contaminated, contact safety team.
3. Dispose of unused dry media through CHATS.
Sharp containers must only be filled to what level?
What is ¾ full or to the fill line?
Containers must be closed when not in use. Also applies to red bins and silver pans.
Who should you contact about incidents or near-misses?
Who is the supervisor.
Contract personnel also may have to contact their contract company and follow their reporting procedures as applicable.
True or false: Gloves are removed in the laboratory room prior to exiting into the laboratory corridor.
What is true.
What is the maximum amount of agar plates or liquid volume that may be autoclaved in the 'normal' size metal pans?
What is no more than 1L (~ 20 agar plates, 100 x 15 mm; ~10 agar plates, 140 x 20 mm).
Small pans: No more than 0.5 L (~10 agar plates, 100 x 15 mm; ~5 agar plates, 140 x 20 mm).
How should broken glass be handled:
A. Pick up broken glass with gloved hands
B. Use mechanical means such as tongs or dustpan and broom
What is B- Always use mechanical means such as tongs or dustpan and broom.
Biological spill kits on each floor have dustpan, broom, tongs, sharps containers. Located by green chemical spill cabinet.
Small pieces can be placed in sharps container, large pieced placed in unlined metal pan and label top of lid with autoclave tape labelled 'Broken Glass'.
Who may receive emergency first aid services at the occupational health clinic?
Who is everyone! (Includes federal employees, contractors and non-federal employees, and visitors)
Bldg 16, Rm 1105; 8am-430 pm; 639-3385
If potential exposure occurs, post exposure evaluation will be conducted, including source testing for HIV/HBV/HCV infectivity
You want to use a new disinfectant for work with human serum that is not currently being used by the lab. Which of these statements is correct:
A. It is ok to use it immediately after verifying on the label that it kills bloodborne pathogens such as HIV, HBV, HCV.
B. You must contact safety team to have the disinfectant approved by OLSS prior to using.
What is B. Disinfectants must be approved by OLSS prior to lab use.
Current approved list:
AF3 wipes, bleach germicidal wipes, Coverage Spray TB Plus RTU, 10% bleach from minimum 5.25% sodium hyphochlorite stock solution, Clorox Hydrogen Peroxide Disinfecting Cleaner RTU
Name three items that are required to prepare waste from the red bins to be autoclaved.
What are a BagPipe, admin label, autoclave tape, cable tie.
•No wire twist ties or autoclave tape to secure BagPipe
•Do not fill bags more than 2/3 full and double bag soft waste in red bins. BagPipe is inside both bags
•No twisting of the inner bag
The lab has several tools available for opening vials or ampules. Name one.
What is ....
Ampule openers (BreakSafe, SnapIT Trolley, AO+, scoring knife)
Vial openers (Decrimper, decapper, hemostat forceps)
Location of glass opener kits:
–B23- storage room – 439
–B18- 1-212 main lab
–B18- B-424B- (ampule openers only, no vial openers except for the hemostat forceps to pick up broken glass)
In the event of an exposure incident involving the skin, eyes, nose, or mouth, what is the length of time that you should flush the contaminated area?
What is 15 minutes?
For percutaneous injury, squeeze around the wound to encourage bleeding while flushing.
Use soap and water for skin.
Remove contact lenses while flushing if applicable.
True or false: Earpods and bluetooth ear pieces are allowed to be worn in the lab.
What is false.
Listening devices are prohibited in the lab area but may be used in the office area.
True or False: Intact vial ampules, Pasteur pipettes, and slides are disposed of in sharps containers.
What is false.
Dispose of in metal pan (with bag) as hard waste.
How do you dispose of metal caps from vials?
A. Sharps container
B. Metal pan as hard waste
C. Regular trash can
What is A.
What is the maximum time period to report a laboratory near miss in the NCIRD NCE database after discovery?
A. 5 working days
B. 2 working days
C. Within 30 minutes
What is B. 2 working days.
You bring your cell phone in the lab and receive a call while you are wearing gloves. What should you do before using it?
What is doff your gloves and wash your hands before use. Use in corridor if possible.
What is the maximum amount of bleach that can be autoclaved according to CDC guidance?
What is up to 50 ml of no more than 10% bleach per autoclave pan is permissible to be autoclaved.
Which letter correctly describes how to prepare sharps containers for autoclaving following disinfection of the outer container?
A. Place on its side in metal pan lined with autoclave bag and cover with metal pan lid.
B. Place in red bin and secure with BagPipe.
C. Place the sharps container in an empty metal pan without a lid, sitting upright. Secure top of sharps container with autoclave tape and place an admin label on the front.
What is C.
Precaution C described in the exposure control plan involves the use of what piece of equipment?
What is a centrifuge.
Precaution C tasks requires the use of a gasket-sealed, interlocked centrifuge with a gasket-sealed rotor or safety cups, and the rotor or cups are to be loaded and unloaded in a biosafety cabinet or similar containment device.
True or false: Non-laboratory incidents are reported in the NCE database.
What is false.
Only laboratory incidents are reported in the NCE database.
Report non-laboratory incidents to the supervisor and follow the DBD job aid in the emergency response folder.