What is a Mycobacteria that doesn't grow in vitro.
100
Mycolic acids (lipids)
What is the cellular component in the cell wall of Mycobacteria that with stands gram staining.
100
This is the capacity to withstand intracellular digestion inside Macrophages
The pathogenicity of Mycobacterium tuberculosis
100
NALC
What is the mucolytic agent in the digestion and decontamination procedure?
100
aerosol transmission
What is route of transmission for M. TB
200
Photochromogen
What is an organism that is buff without light while the pigment intensifies when exposed to light?
200
Pink
What is the color of M. tuberculosis when stained with the acid fast stain
200
The mycobacterial species associated with disseminated disease in AIDS patients
What is M.avium complex (MAC)
200
a 24 hour pooled urine
What is an unacceptable specimen for mycobacterial culture
200
An element of safety in any laboratory regardless of the level of service provided. This test is performed annually on microbiology personnel.
What is Tuberculin skin testing
300
A rapid grower
What is a category of Mycobacteria that grows on culture media within 7 days
300
Kinyoun Stain
What is the acid fast stain method referred to as the "cold acid fast stain"
300
niacin and nitrate positive, catalase positive, heat stable catalase negative.
What are the biochemical reactions for M.TB
300
sterile, non-viscous fluids
What is a specimen that does not require digestion and decontamination procedures
300
Characterized by the formation of cheese-like necrosis and lesions in the lungs.
What is the infection due to tubercle bacilli (MTB)
400
The colony morphology of M. tuberculosis
What is a slow growing, rough, buff, non-chromogenic colony.
400
unstained bacilli or beaded bacilli
What is the appearance of M.TB. when stained with the gram stain
400
Development of disease in lab mice after inoculation of the organism on the foot pad of the mice.
How is M. leprae diagnosed
400
A first morning sputum is received for culture of mycobacteria. It is digested and concentrated by the N-acetyl-L-cysteine alkali method. Two Lowenstein-Jenson slants are inoculated. One is incubated in the dark at 35C with 5-10% CO2 and the other in light. The smears reveal acid-fast bacilli and after 7 days no growth appears on the slants. The best explanation is:
What is insufficient incubation time
400
Centrifuge to concentrate and prepare smear and inoculate media with sediment
What is the concentration procedure used for sterile, non-viscous fluids
500
Scotochromogen
What is a colony that appears as an orange-yellow colony in light and without light.
500
serpentine cording
What is the unique staining characteristic of M.TB which appears as a rope or cord arrangement when stained with the acid fast stain
500
large numbers of AFB found in lesions but organism is unculturable on routine mycobacterial media.
What is lepromatous leprosy
500
Lowenstein-Jensen
What is an egg-based medium used for cultivation of mycobacterium
500
increased contrast between the organism and the background, improved sensitivity compared to the Kinyoun or Ziel Neelson acid fast stain.
What is auromine rhodamine fluorescent stain for Mycobacteria