Retinal cell atlases
The scientific name of this specie is Mus Musculus.
What is a Mouse?
These are expressions of class markers that have been validated in mice and primates.
What are genes that are co-expressed within a retinal cell class but exhibit little or no expression in other retinal cell classes.
These are three of the retinal cell classes that have been subdivided into subclasses.
What are photoreceptors comprise rods, specialized for low-light vision, and cones?
The number of bipolar cell types in mice.
What is 15?
The number of RGC orthotypes identified in mammals.
What are 21 RGC orthotypes?
The number of nuclei from 18 human donors.
What is 185,000 nuclei?
This was used to compare interspecies similarity of classes.
What is Pseudobulk?
Nearly all amacrine cells use this as their neurotransmitter.
What is either GABA (γ-aminobutyric acid) or glycine?
This is a non-canonical OFF bipolar cell.
What is BC1B or GluMI?
The percent of mammalian RGC clusters mapped predominantly to a single orthotype.
What is 81%?
The sequences used to study retinal cell types in 5 species.
What is scRNA-seq and snRNA-seq?
This analysis found that transcriptomic similarity was driven by cell class identity rather than species identity.
What did a cross-correlation analysis of 16 jawed vertebrae find?
These cells can be subdivided into those that depolarize and hyperpolarize to illumination—ON and OFF types .
What are Bipolar cells?
This data revealed a number of groups based on shared transcriptomic signatures?
What are 14 groups of cells?
This is the extent of variation within cell classes increases systematically from outer to inner retina in order.
What is photoreceptor < horizontal cell < bipolar cell < RGC?
The scientific name of this specie is Petromyzon Marinus.
What is a Sea Lamprey?
These are changes in cell-type composition as well as from changes in gene expression in individual cell types.
What are variations at the pseudobulk level?
These genes are expressed selectively in mouse retinal subclasses.
What are NRL and NR2E3 in rods, THRB and LHX4 in cones, MEIS2 in GABAergic amacrine cells, TCF4 in glycinergic amacrine cells, FEZF2 and LHX3 in OFF bipolar cells, and ISL1 and ST18 in ON bipolar cells?
These sometimes contain small sets of related types.
What are neuronal orthotypes?
This is the amount of hours from death that human eyes were obtained.
What is a median of 6 hours?
These cells were not analyzed.
What are microglia or endothelial cells?
This trend was assessed by comparing mean squared expression divergence in pseudobulk profiles and evolutionary distance among pairs of species for each cell class.
What are evolutionary trends?
This suggests that programmes specifying subclasses, like those specifying classes, are evolutionarily ancient.
What is subclass-specific expressions?
These are two biopolar cell orthotypes.
What are oRBC and oBC1B?
The question we all have now after going through this jeopardy.
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