Lab Ettiqute
Where do used gloves go?
The red biohazard bin
two genes required to convert zeaxanthin into capsanthin and capsorubin
ZEP and CCS
What is the function of a plasmid in genetic engineering?
:)
Name of a reaction that amplifies DNA
PCR
DNA separates by size
Why is it important to keep enzyme stocks on ice?
Enzymes are temperature sensitive and warm temperatures may damage stock solutions
Name one real life use of carotenoid pigments
Food dye (beta carotene in Celsius energy drink)
or
Carotenoids absorb excess light energy in photosynthesis
Describe the role of the pBAD vector in our spring semester experiments
Inputs into a gibson assembly reaction
linear backbone and linear inserts with ~20bp overlaps
what did all our fall experiments have in common
removing or making a gene non functional
Why do we use NF water rather than regular water?
NF water is Nuclease Free!
Name a place on the internet were DNA can be purchased
IDT, TWIST, ADDGENE
Purpose of an inducible promoter
control gene expression
what is unque about the primers used in an inverse PCR
They overlap with each other
common mistake students make when picking bacterial colonies
how should you hold an enzyme reagent
what was the original plasmid we ordered called and what did it produce
name a part you need in a plasmid
antibiotic resistance, ori, Gene of interest, promoter, terminator
purpose of a glycerol stock
preserve bacteria for future experiments
Why do we perform a differential digest before sequencing our clones?
serves as a "gut check", avoids wasting money on sequencing
When do we use a bunsen burner in lab and why
Aseptic technique for anything that can get icky like outgrowth media or LB plates
What's the difference between zeaxanthin and zeaxanthin diglucoside
zeaxanthin diglucoside is water soluble
name the 3 steps to make a lot of a plasmid you cloned and extract it
transform -> LC -> miniprep
Two components of a restriction enzymes functionality
recognition site and cut site
role of DpnI
get rid of template plasmid