Media mastery
Name that enzyme
Colony characteristics
Antigen ally
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100

This agar contains crystal violet and bile salts to inhibit Gram-positive organisms

What is MacConkey Agar

100

This enzyme is what causes the pink color to colonies and agar on the MAC 

What is lactase

100

This genus is nonmotile and displays mucoid colonies 

What is Klebsiella

100

This antigen is known as the somatic antigen

What is O antigen

100

This species TSIA is A/A with gas and citrate negative

What is Escherichia coli

200

This is the pH indicator in the citrate test

What is bromthymol blue
200

This enzyme breaks down urea into ammonia and CO2, turning the medium pink

What is urease

200

This genus is known for displaying swarming motility on BAP

What is Proteus
200

This antigen is known as the flagellar antigen

What is H antigen

200

This test detects H2S production, indole and motility 

What is SIM

300

This agar is used to differentiate Salmonella and Shigella from other normal flora enterics

What is XLD agar
300

This enzyme is responsible for DNA hydrolysis and is positive in Serratia marcescens

What is DNase

300

This genus may produce a brick red pigment on MAC and can be confused as lactose positive

What is Serratia

300

This antigen can interfere with the detection of the O antigen

What is K antigen

300

In the TSIA which sugar is on the top and which is on the bottom

What is lactose on top and glucose on bottom

400

Which media is the most sensitive for H2S production

What is SIM

400

This enzyme is detected in the ONPG test

What is Beta-galactosidase


400

This species shows a metallic green sheen on EMB agar 

What is Escherichia coli


400

What antigen does Klebsiella lack due to being nonmotile in nature

What is H antigen

400

Which organisms test positive in PDA test

What is Proteus, Providencia, Morganella

500

This reagent must be added to PDA test after incubation

What is ferric chloride

500

This enzyme is detected using bromocresol purple as the pH indicator

What is amino acid decarboxylase

500

This genus may be confused with Klebsiella due to its capsule production and forms pink colonies on MAC

What is Enterobacter

500

This antigen is unique to Salmonella Typhi and contributes to its virulence

What is Vi antigen

500

This genus is PDA positive, citrate positive, and TSIA- K/A 

What is Morganella