Pathmaker

DNA

100

What does DNA stand for 

What is deoxyribose nucleic acid?  

200

DNA is has what charge?

What is negative?

300

Large pieces of DNA are found where on a gel? Why?

What is Top because large pieces of DNA cannot fit through the microscopic holes in the gel.

400

What does PCR stand for and what does it do

Polymerase chain reaction, a reaction that copies one strand of DNA into one billion

500

DNA makes RNA makes ___________

What is protein

Safety

100

These three pieces of PPE are always required in the lab

What are gloves, goggles, and lab coat

200

Gloves get thrown away in the _______

What is biohaz

300

True or False: You should always pH with a friend. Why?

What is true! Because often times the acids and bases you are working with are very strong and dangerous. A friend should be around in case anything goes down

400

DAILY DOUBLE
A _____provides detailed information about a hazardous chemical's properties, health hazards, protective measures, and safety precautions for handling, storing, and transporting 

Safety Data Sheet (SDS)

500

Oh no! You spilled a chemical. Whats the first thing you should do?

What is tell a lab manager

Pipetting

100

When do you go down the the first stop?

What is when aspriating

200

True or false? You can set a pipette out of range and it will  be totally fine

What is false!

300

What is the range of a p100 pipette

10 uL - 100 uL

400

How would you measure 657 uL

What is using a p1000.

500

How would you measure 7.4 mLs?

What is using a 10 mL serological to measure 7 mLs and a p1000 to measure 400 uL

OR 

What is using a 10 mL serological to measure 7.4

Aseptic Technique

100

True or false? You should never touch your face or phone with your gloves

What is true! If you've seen Maddie or me touch my phone with my gloves no you havent!

200

You should always clean with this before starting an expiriment

What is 70% isopropanol or ethanol

BONUS QUESTION

Why 70% and not 100%

300

How many times should a serological tip be used

What is once

400

If you need to put a cap down on you bench which way should you do it?

What is face up?

500

How do you aseptically enter a BioSafety Cabinet (BSC)

What is spray down the cabinet, spray down you arms, and make sure not not block the fan?

Math

100

This equation is used to dilute liquid stock

What is C1V1

200

M=?

What mols/liter

300

I have a stock of 5M tris buffer. I want 1 liter of 400mM tris buffer. How would I make this?

What is measure 80 mLs of stock in a graduated cylinder and QS to 1 L with water. 

400

I want to make 1L of a 4M NaCl solution

NaCl FW: 58.44

How would I make this

What is use a scale to measure 233.76 grams of NaCl, stir that with about 500 mLs of water until dissolved, and QS to 1 L.

500

I want to make a 1L buffer thats 1M tris and 0.5M NaCl

My tris stock is 5M

NaCl FW: 58.44

How would I make this

What is weigh 29.22 grams of salt on scale. Then dissolve in about 500 mLs of water. Put this in a graduated cylinder. Add 200 mLs of tris stock. QS to 1 L