What Is It?
History
Fun Facts
100

What does PCR stand for?

Polymerase Chain Reaction

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Who discovered it?

Kary Banks Mullis

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What are some applications?

Genotyping, cloning, mutation detection, sequencing microarrays, forensics, and paternity testing

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What is its function?

It creates multiple copies of a specific segment of DNA

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What year was is discovered?

Between 1983-1985

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How long did this whole process take?

14 years

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How does it work? 

Using short fragments of DNA, they rapidly amplify the DNA replication process

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How was it discovered?

Kary was trying to improve the synthesis of oligonucleotides. He envisioned the concept of PCR on a camping trip in 1969. 

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What are the 3 different stages?

1. Denaturing 

2. Annealing 

3. Extending