History of PCR
Who invented PCR
Who is Kary Mullis?
What does PCR stand for?
Polymerase Chain Reaction
What is denaturation in PCR?
What is heating DNA to separate its strands?
How is PCR used in medical diagnostics?
What is detecting pathogens like viruses and bacteria with high specificity?
What is the main risk of contamination in PCR?
What is leading to false positives or misdiagnoses?
What inspired Kary Mullis to create PCR
What is a Disease that killed his friend?
What is the main purpose of PCR?
To copy specific DNA sequences.
At what temperature does annealing occur?
What is around 50–60°C?
Name one use of PCR in forensic science.
What is amplifying DNA evidence for identification?
What is one ethical concern in forensic PCR?
What is the potential misuse of genetic information, causing privacy issues?
What enzyme made PCR more efficient?
What is Taq polymerase?
What are the three key steps of PCR?
What are Denaturation, Annealing and Extension?
What is the role of primers in PCR?
What is binding to DNA templates to provide starting points for synthesis?
How does PCR contribute to genetic research?
What is aiding in studying mutations, gene expression, and cloning?
Why is PCR less accessible in low-resource areas?
What is the high cost of technology and reagents?
In what year did Kary Mullis win the Nobel Prize?
What is 1993?
What equipment is crucial for performing PCR?
What is a thermocycler?
What happens during the extension step?
What is Taq polymerase adding nucleotides to primers, creating new DNA strands?
How is PCR used in agriculture?
What is detecting GMOs and developing disease-resistant crops?
How does PCR impact the environment?
What is generating lab waste and altering ecosystems through genetic engineering?
Why is Taq polymerase essential to PCR?
What is It is heat-resistant?
How does DNA amplification grow in PCR?
What is exponential growth, doubling with each cycle?
How many cycles are typically used in PCR?
What is 20–40 cycles?
What is real-time PCR used for?
What is tracking infectious diseases and providing public health data?
What are the dual-use concerns of PCR?
What is the potential misuse for creating biological weapons?