Components of PCR
The Original DNA To Be Copied
What is Template DNA?
Depends on the composition of primers, between 50-65 degrees
What is the temperature for annealing?
the Best Choice for Amplifying DNA
Maintains Optimal PH
What are Buffers?
72 Degrees
What is the optimal temp for extension?
It is used to Synthesize DNA
What is DNA Polymerase
Building Blocks of the PCR Reaction
What are Nucleotides?
when the two DNA strands separate because the hydrogen bonds between the complementary bases are broken by the high heat
What Is Denaturing?
Includes buffer, magnesium, nucleotides and TAQ polymerase
What are the ingredients of the Master Mix?
Adds Nucleotides to Synthesize New DNA Strands
What is Taq Polymerase?
When the temperature cools, the forward and reverse primers bind before and after the target sequence
What is Annealing?
it tells the researcher that the PCR reaction and sequencing reactions will give correct results
What are Positive Control PCR Tube?
Attach before and after the target sequence to locate the sequence to be amplified
What are Primers?
Taq polymerase adds and bind nucleotides to build new DNA strands
What is Extension?
A heat resistant polymerase found in bacteria that live in hot springs
Taq Polymerase