Background
What gene makes the bacteria glow green?
GFP gene
What step involved heating the tubes to 42°C?
Heat shock
Did the bacteria glow without arabinose?
No
What sugar turns the glowing gene ON?
Arabinose
What technique prevents contamination?
Aseptic technique
Why did bacteria NOT grow on LB + Amp plates with no pGLO?
Because it lacked ampR, ampicillin killed the cells.
How does this experiment show the relationship between genotype and phenotype?
Only bacteria that received the pGLO plasmid (genotype) could glow when arabinose was present (phenotype).
What chemical helped make the bacteria able to take in DNA?
Calcium chloride (CaCl₂)
Which plate glowed and why?
LB + Amp + Arabinose + pGLO, because arabinose turned on GFP