Infection Control & PPE
What is the most effective way to prevent the spread of infection in a healthcare setting?
Proper hand hygiene
Which test is most commonly performed in a lavender tube?
CBC (Complete Blood Count)
Which department analyzes glucose, cholesterol, and liver panels?
Chemistry
What is the proper angle of insertion for a venipuncture needle?
15–30 degrees
You enter a patient’s room to draw blood. The patient is asleep. What should you do first?
Gently wake the patient and confirm their identity using two identifiers.
Which type of PPE must always be worn during a blood draw?
Gloves
What test is performed to monitor Warfarin therapy?
Prothrombin Time (PT)
What federal regulation sets lab testing standards for accuracy and reliability?
CLIA
What is the correct order of draw for blood collection tubes?
Blood Cultures → Light Blue → Red/Gold → Green → Lavender → Gray
While performing venipuncture, the patient says they feel faint. What should you do immediately?
Stop the procedure, lower their head or lay them down, and ensure their safety.
What is the correct order for donning PPE (putting on)?
Gown → Mask/Respirator → Goggles/Face Shield → Gloves
Which test requires a gray-top tube and is sensitive to glycolysis?
Glucose or Lactic Acid
What organization accredits healthcare organizations and enforces National Patient Safety Goals?
The Joint Commission
What is the recommended site for heel stick collection on a newborn?
Lateral plantar surface of the heel
You attempt a blood draw but no blood flows into the tube. You’ve confirmed the needle is in the vein. What is your next best step?
Try slightly repositioning the needle or change the tube in case of vacuum failure.
What does the chain of infection require in order for infection to spread?
An infectious agent, reservoir, portal of exit, mode of transmission, portal of entry, and a susceptible host
What tube and department would you send for a Type & Crossmatch?
Pink tube; Blood Bank
Which department would receive a urine culture for analysis?
Microbiology
What is the clean-catch midstream urine method used to diagnose?
Urinary Tract Infections (UTIs)
You’re about to draw a lavender, green, and red tube. You only have one clean needle left. What do you do?
Follow the correct order of draw: red → green → lavender using the same needle, but change the tubes as required.
A phlebotomist accidentally splashes blood into their eyes. What is the immediate first step they should take?
Rinse eyes with water or saline for at least 15 minutes and report the exposure immediately
Which tube is required for a blood culture and what is the additive?
Yellow SPS; Sodium Polyanethol Sulfonate
What document must be signed by the patient before releasing their health information?
HIPAA Authorization Form
What is another name for the newborn screening PKU test?
Guthrie Test
During a draw, blood splashes on your glove and skin through a tear. What steps should you take immediately?
Remove gloves, wash the area thoroughly with soap and water, report the incident to your supervisor, and fill out an exposure report.