Additives
Order of Draw
Safety Scenarios
Collection
Gov. Agencies
100

What additive is in the red glass top tube?

None

100

What comes first in order of draw?

Blood Cultures

Aerobic (blue top), Anaerobic (purple top)

100

OSHA requires that a sharps container be changed when? 

3/4 full

100

What color tube has Sodium Citrate and what department does it get sent to?

Light Blue

Coagulation Dep.

100

HIPAA

Health Insurance Portability and Accountability Act - patient confidentiality

200

What is in the tiger top tube?

Clot activator and thixotropic gel for serum separation.

200

What tubes comes after the grey tube? 

Royal blue and tan.

200

An open container is spilled on the floor in the clinic waiting area, what should the phlebotomist should use?

Material Safety Data Sheet

200

What is a PSA test and what tube is it collected in?

Prostate Specific Antigen, collected in a plasma or in an SST (gold)

200

OSHA

Occupational Safety and Health Administration - workers safety.

300

What is in the Pink and Purple tubes?

EDTA

300

Recite the order of draw

Blood cultures, Lt. Blue, Red, Gold, Lt Green, Dk Green, Lavender, Gray, Yellow

300

What is the first and most important thing to do if the phlebotomist sustains a sharps injury? 

Wash puncture and small wounds for 15 minutes.

300

What does the Royal Blue tube test?

Used in the collection of whole blood or serum for trace element analysis.

300

CLSI

Clinical Lab Standards Institute - sets standards for labs.

400

What is in the orange tube?

Thrombin 

400

What group of tubes come third in the order of draw and are considered serum tubes? Give the common lab tests for these tubes.

Red, gold, tiger top - chemistry, serology, immunology

400

After a venipuncture, a patient experienced serve nerve damage and lost feeling in their fingers, what actions by the phlebotomist most likely occurred?  

Incorrect needle angle was used

400

What is a BMP test and what tube is it collected in?

Basic Metabolic Panel, Gold SST or mint green Lithium Heparin PST

400

CLIA

Clinical Lab Improvement Act - amendment certifies labs.

500

Additives for microtainers? Give the chemical formula.

K2 EDTA (lavender), lithium heparin (dark green), lithium heparin and gel (PST, mint green), NaFl/Na2 EDTA (grey), SST gel (gold), none (red)

500

Order of draw for microtainers? 

lavender, green, mint green + mint green amber tube, grey, gold + gold amber tube, red

500

A phlebotomist in a doctors office needs to perform  venipuncture on a patient who had been diagnosed with SARS, which transmission based precaution should the phlebotomist take?  

Droplet

500

When ordering a PSA, CBC, ETOH, and PT, in what order would you draw? Give the correct color tube and their additive.

Light blue (sodium citrate), gold SST (clot activator and gel), lavender (KEDTA), gray (potassium oxalate, sodium fluoride).

500

JCAHO

Joint Commission - for the Accreditation of Healthcare Organizations (advocating for safer health care)