Blood Volume
Blood Volume of a Newborn
90 mL/kg
Define Hemolysis
Bonus: what is it's affect on plasma samples?
breakdown on RBCs which leads to leakage of HgB
Plasma specimen will turn pink (should be pale yellow color)
List Four (4) Contraindications for Capillary Puncture
Dehydration, Shock, Cold or Mastectomy Side
What test type uses a clot activator?
Bonus Point for color of the tube
Serology (Serum)
Red Tube
SPS
Yellow, Blood Culture
Blood Volume of an Adult
70 mL/kg
What temperature should a warming device not exceed?
42°C or 107.6°F
What is the device used for capillary puncture?
Lancet or Laser Lancet
Which anticoagulant inhibits thrombin formation?
Heparin
Citrate
Light Blue, Coagulation
Blood Volume of a Infant
80 mL/kg
Define Petechiae (& cause)
tiny, non raised red spots that appear on the patient’s skin when a tourniquet is applied too tight
Sites of Capillary Puncture
Adults: Middle or Index (ring) Finger
Infants: Heel (medial or lateral plantar surfaces)
How do the reference values are differ in Venous and Capillary Blood?
Capillary Blood: high in Hemoglobin & Glucose
Venous Blood: high in Proteins & Calcium
Heparin
Green, Chemistry
Calculate the Total Blood Volume and Safe Withdrawal of a 150 pound adult
Answer: 4,760mL & 142mL (3%)
Math:
150 / 2.2 = 68 (to find kg)
68 x 70 = 4,760 (kg x blood volume to find total blood volume)
4,760 x 0.03 = 142 (to find 3%)
What are the 4 blood types?
Type A, B, AB & O
What is Newborn/neonatal screening & its importance?
State mandated testing of newborns for presence of genetic, metabolic, hormonal & functional disorders for early diagnosis and treatment for better health
Place these blood tests in order and identify tube color:
HgB, FBS, BUN, Blood Culture, PT
Blood Culture (yellow), PT (light blue), BUN (red), HgB (lavender), Glucose (gray)
EDTA
Lavender, Hematology
Calculate the Total Blood Volume and Safe Withdrawal of a 6 pound Newborn
Answer: 245mL & 7mL (3%)
Math:
6 / 2.2 = 2.7 (to find kg)
2.7 x 90 = 243 (kg x blood volume to find total blood volume)
243 x 0.03 = 7.29 (to find 3%)
What is bilirubin & what color tubes should be used during testing?
Product of the breakdown of RBCs, deposits of yellow bile pigment in the skin and eyes (jaundice).
Amber or brown colored tubes (or wrapped in aluminum foil) because it's light sensitive.
What is the capillary blood?
A mixture of venous and arterial blood; more arterial blood when warmed.
Difference between Vascular & Avascular
Vascular = tissue that contains blood vessels
Avascular = tissue that does not contain blood vessels
Oxalate
Gray, Glucose