Definitions
Voltage
What is the separation of opposite charges?
At 30C, the equilibrium potential of sodium when you have 40mM extracellularly and 200mM intracellularly
What is -41.9mV?
At 30C, the membrane potential when the membrane is equally permeable to Na, K, and Cl- and:
[K+]out = 4mM, [K+]in = 150mM
[Na+]out = 145mM, [Na+]in = 15mM
[Cl-]out = 110mM, [Cl-]in = 10mM
What is -14.3mV?
The approximate resting potential range of neurons
What is -30 to -90mV?
Membrane potential
What is the difference in electric potential between the interior and exterior of a cell?
At 30C, the equilbrium potential of Ca2+ when you have 300mM extracellularly and 25mM intracellularly
What is +32.4mV?
The form of RNA used as interference for knock down
At 30C, the membrane potential when the membrane is equally permeable to Na, K, and Cl- and:
[K+]out = 58mM, [K+]in = 234mM
[Na+]out = 254mM, [Na+]in = 13mM
[Cl-]out = 456mM, [Cl-]in = 23mM
What is -19.4mV?
The conductance across the phospholipid bilayer with no channels
What is 0?
Equilibrium potential
What is the membrane potential when there is no net movement of ions across the phospholipid bilayer?
At 25C, the equilibrium potential of potassium when there is 49mM extracellularly and 678mM intracellularly
What is -67.5mV?
2 strengths of using CRISPR over other options
Using multiple genes at the same time
Can control point mutations and conditional alleles
At 30C, the membrane potential when the membrane is equally permeable to Na, K, and Ca2+ and:
[K+]out = 159mM, [K+]in = 13mM
[Na+]out = 26mM, [Na+]in = 100mM
[Ca2+]out = 151mM, [Ca2+]in = 90mM
What is +1.06mV?
The person who came up with the equation to determine equilibrium potential for one ion
Who is Nernst?
What is a change that makes the membrane potential more negative?
At 15C, the equilibrium potential of chloride when there is 389mM extracellularly and 234mM intracellularly
What is -12.6mV?
Enzymes used with RNAi knockdown
What are dicer and RNA-dependent RNA-polymerase (RdRP)?
At 40C, the membrane potential when the membrane is equally permeable to Na, K, and Ca2+ and:
[K+]out = 234mM, [K+]in = 54mM
[Na+]out = 48mM, [Na+]in = 727mM
[Ca2+]out = 34mM, [Ca2+]in = 272mM
What is -10.4mV?
The component of the membrane that establishes the sodium and potassium gradients
What is the sodium potassium pump?
Driving force
What is the net electromotive force that acts on an ion to cause it to cross the membrane?
At 45C, the equilibrium potential of Mg2+ when there is 600mM extracellularly and 450 intracellularly
What is +3.94mV?
What are crRNA, tracrRNA, CRISPR, Cas9, and PAM?
At 20C, the membrane potential when the membrane is equally permeable to Na, K, and Ca2+ and:
[K+]out = 161mM, [K+]in = 89mM
[Na+]out = 56mM, [Na+]in = 940mM
[Ca2+]out = 163mM, [Ca2+]in = 34mM
What is -3.94mV?
ATP is removed from the inside of a neuron. What happens?
Resting potential would change because the sodium potassium pump would not be able to maintain the ion gradients