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Go Cycling!
History Lesson
Process
100
Cycle 1 creates this many copies of target DNA
What is 2?
100
The creator of PCR
Who is Kary B. Mullis
100
The number of steps in a cycle
What is 4?
200
Cycle 2 creates this many target DNA
What is 4?
200
The name of the prize awarded to Mullis
What is the Nobel Prize for Chemistry
200
The step when strands of DNA are separated into single strands
What is denaturation?
300
Eight copies of target DNA are made in this cycle
What is cycle 3?
300
PCR is also called this
What is molecular photocopying?
300
The temperature does this in the anneal primers stage
What is lower?
400
There will be 1, 073, 741, 764 target DNA copies at the end of this cycle
What is cycle 30?
400
The technique of amplifying is also called...
What is copying?
400
The role of the enzyme, taq Polymerase in the third step
What is extend/synthesize a new strand
500
This is the type of growth the target copies experience
What is exponential?
500
PCR is amplifying these tiny pieces
What are DNA segments?
500
The number of times the cycle repeats
What is 30 to 40 times?