Applications
The goal of PCR?
Replicate sections of DNA
The person who invented PCR won the Nobel prize in this category
Chemistry
The first step of PCR
Denaturing
The type of bonds broken during Denaturing
Hydrogen Bonds
The reason replicating DNA is important
Allows DNA to be viewed and manipulated
The person who invented PCR
Kary Mullis
The number of times Annealing, Extension and Denaturing happens during PCR
30-40 times
The approximate number of DNA copies created after PCR
~1 billion
The process used to visualize the results of PCR
Gel electrophoresis
The person who synthesized Taq polymerase from thermophilic bacteria
Alice Chien
Device used to regulate temperatures during PCR
Thermocycler
The reason Taq Polymerase is used during PCR
High heat stability
A downside of using PCR
Can only detect known DNA sequences
or
Complicated process can lead to errors resulting in false positive tests
The year PCR won a Nobel Prize
1993
The rate DNA replicates during PCR
Exponentially
The approximate length of DNA primers used during PCR
20 nucleotides
The reason law enforcement uses PCR
To conduct genetic testing to match DNA sequences
The year PCR was invented
1985
The temperature denaturing takes place (oC)
96oC
The temperature that Taq Polymerase needs to be at to extend primers
72oC