Restricción
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Who dunnit?
Genetic Engineering
100

When a restriction enzyme makes a cut that only breaks phosphodiester bonds, it's called ...

What is a blunt end?

100

PCR is an acronym for ___________ ___________ __________.

What is polymerase chanin reaction?

100

The gel used in electrophoresis is usually made of _______ ... a naturally occuring substance in the ocean.

What is agarose?

100

DNA fingerprinting deals with repetitive sequences in the ___________ portion of ____.

What are non-coding and DNA?

100

A plasmid is a very common ____________ (delivery mechanism) used in genetic engineering.

What is vector?

200

Restriction enzymes occur naturally in ______________ as defensive mechanisms against ________ DNA.

What are bacteria (prokaryotes) and viral?

200

The machine used to carry out the process of PCR is a ________________.

What is a thermocycler?

200

DNA is ________________ charged ... mainly because of the ______________ in each nucleotide.

What are negatively and phosphate?

200
When examining a restriction map, offspring will have all or some of the same bands as the mother?

What is some?

200
Nucleic acid polymer that contains genetic material from more than one organism is described as ____________.

What is recombinant?

300

Restriction enzymes belong to this macromolecule group and are constructed out of ___________ ______.

What are protein and amino acids?

300

_____ polymerase is used during the extension stage of PCR ... it's selected for use because it won't ____________ at high temperatures.

What are Taq and denature?

300

The ___________ is used in the first lane of the gel and is composed of fragments of known length.

What is ladder?

300

Famous detective created by Arthur Conan Doyle ...

Who is Sherlock Holmes?

300

Organisms that contain recombinant DNA are referred to as _______________.

What is transgenic?

400

Restriction enzymes cut at _______________ sequences, which are described as ______________ (grammar term).

What are recognition and palindromes?

400

_______ bonds are broken during the first stage of PCR, know as ________________.

What are hydrogen and denaturation?

400

Having positive and negative poles on eother end of the gel electrophoresis device creates an ________________ _____________.

What is electrical field?

400

Name of original team lead on NCIS, played by actor Mark Harmon ...

Who is Leroy Jethro Gibbs?

400

Enzyme responsible for 'joining' vector with foreign section of DNA ... like a glue stick

What is DNA ligase?

500

Superman is to kryptonite as restriction enzymes are to __________ __________.

What are methyl groups?

500

_______________ ____________ invented PCR.

Who is Kary Mullis?

500

The name of the company that we bought our gel electrophoresis devices from is ....

What is miniPCR?

500

Famous female mystery novelist, ____________ Christie.

Who is Agatha?

500


What is Finn Dorset?