Machine used to cut thin sections of the tissue from the wax cube
What is Microtome
100
Distance at which two objects must be separated to see them as two distinct objects
What is Resolution
100
form salt linkages with oppositely charged tissues
What is ionic dyes/stains
100
high magnification
What is 400
200
The use of a microscope
What is Microscopy
200
The five steps to the paraffin method of tissue processing
What are 1. collecting the sample
2. Fixation
3. Dehydration, Clearing, and Embedding
4. Sectioning
5. Mounting and Staining
200
Has a resolution of about 0.2micrometers, or about the size of a mitochondrion
What is light microscope
200
hematoxylin staining blue/purple coloration, described as basophilia stains these structures
What are nucleus (heterochromatin and nucleolus), ribosomes, rough endoplasmic reticulum
200
100
What is low magnification
300
The study of cells and organelles
What is Cytology
300
During the fixation process, formalin, an aqueous solution of formaldehyde, does this
What is coagulates and hardens proteins to prevent enzymatic activities preventing postmortem degeneration while preserving architecture of tissues.
300
Resolution at which you are able to see a ribosome or cell membrane and with what
What is transmission electron microscope (TEM). Resolution is 0.1 nm or 1 angstrom
300
Eosin stains pink/red and is described as acidophilia or eosinophilia stains these structures
What are most proteins, cytoplasm, cytoplasmic filaments, secretory vesicles, collagen, elastic fibers
300
1000
What is oil immersion power
400
Uses special chemicals to identify tissues, cells, and cellular components
What is Histochemistry/cytochemistry
400
Clears alcohol from sample
What is xylol or toluol
400
Resolution of the human eye
What is 0.2mm. or about the size of an oocyte
400
Used to describe components that stain blue with the basic dye hematoxylin
What is basophilia or base-loving
400
Observable characteristics of a tissue that would not have been present in real life but are the result of the preparation and staining processes.
What are artifacts.
500
necessary to view cellular and extracellular components
What is staining
500
During mounting stage, tissue is rehydrated using this to allow structures to be seen more clearly
What is decreasing concentrations of alcohol
500
Depth of view
What is magnification
500
Special stain used to stain carbohydrates, glycogen, proteoglycans, mucus, basement membrane cellulose. Stains magenta. Used to assist in the diagnosis of some diseases.
What is Periodic Acid Schiff stain (PAS)
500
Artifacts include these
What are postmortem degeneration (not fixed properly), shrinkage, precipitate, wrinkles or folds, microtome knife nicks, and distortion (rough handling).