Protein Purification
The size of protein that comes off a size exclusion column first?
What is big?
The position of hydrophobic amino acids in a folded cytoplasmic protein.
What is inside the protein?
The chemical bond formed between electronegative nitrogen and hydrogen.
What is hydrogen bond?
Translates to "rock" or "cliff"
What is Schemer? (Shkëmber translates to "rock" or "cliff" in Albanian).
The material found at the bottom of a tube following centrifugation
What is a pellet?
The chromatographic technique that relies on the chemical property of a "lock and key"
What is affinity chromatography?
The type of chemical bond responsible for secondary structure.
What is hydrogen bond?
The location of hydrophobic amino acids in membrane protein.
What is outside?
This word means one who is a source of joy or one who brings happiness.
The piece of equipment used to mix a sample in lab.
What is a vortexer?
The chemical used to unfold proteins prior to SDS-PAGE
What is SDS?
The two main secondary structures.
What is alpha helix and beta pleated sheet?
Side-chain interactions between amino and acid groups contribute to this level of protein structure.
What is secondary structure?
Title given to an Arab leader, in particular the chief or head of a tribe, family, or classroom.
What is Sheikh or Dr. Sigma?
Describes the cellular components released following treatment of cells with detergent and lysozyme.
What is size exclusion chromatography?
The three major side chain interactions responsible for tertiary structure
What is salt bridge (ionic bond), disulfide bond and hydrophobic clustering
What is oxygen and nitrogen?
The Irish city where you will find the Blarney Stone?
What is Cork?
The term for the multiple samples collected by traditional column chromatography.
What is lysate?
The chromatographic technique that relies on the chemical property "like dissolves like"
What is hydrophobic interaction chromatography?
The amino acid that forms disulfide bonds
What is cysteine?
The primary secondary structure contributing to the folding of GFP.
What is beta sheet?
The German word for white.
The technical term for the beads in a column.
What is stationary phase?