STRUCTURE
Sequence of amino acids in the protein structure
What is primary structure?
Name of the assay used to determine total protein concentration
What is the Bradford assay?
Method involving sound waves used to break open bacterial cells
What is sonication?
Blue dye used to stain bands on a protein gel
What is Coomassie Blue?
First step of running any Westernblot
What is SDS-PAGE?
Type of bond holding together the beta-sheets and alpha-helices
What is a hydrogen bond?
Reagent used to assay beta-galactosidase
What is ONPG?
Type of IEC resin used to purify beta-galactosidase
What is DEAE-Sepharose?
Dye added to the protein sample buffer for SDS-PAGE
What is bromophenol blue?
Material used as support for the Westernblot
What is nitrocellulose membrane?
Type of non-covalent interaction between charger amino acid side chains
What is an electrostatic interaction?
Name of the yellow reaction product with absorbance at 420 nm
What is ONP (ortho-nitrophenol)?
Type of chromatography used to purify a monoclonal antibody
What is affinity chromatography?
Reagent in the sample buffer that reduces the disulfide bonds
What is Beta-ME (Beta-mercapto-ethanol)?
Buffer used for preventing non-specific binding of antibody during a Westernblot
What is blocking buffer?
Two examples of acidic amino acids which will have negatively charged side chains at pH 7.4
What are Asp and Glu?
Common protein used to create the standard curve in a total protein assay
What is BSA?
Common salt used to precipitate out proteins in an early purification stage
What is ammonium sulfate?
Full name of the reagent added to the sample buffer that ads negative charges to all proteins
What is sodium dodecyl sulfate?
Name of the enzyme linked to the secondary antibody in our Westernblot system
What is alkaline phosphatase?
Name used for a central carbon atom with four different substituent groups
What is a chiral atom?
Beta-galactosidase activity per mass unit of total protein
What is specific activity?
Technique used for industrial scale desalting of protein samples
What is TFF (Tangential Flow Filtration)?
Technique of separating proteins on a gel based on their pI differences
What is IEF (Isoelectric Focusing)?
The two reagents that form the chromogenic substrate for our Westernblot system
What are NBT (Nitro blue tetrazolium) and BCIP (5-Bromo-4-chloro-3-indolyl phosphate)?