Pumps
Which small ion transport mechanism is NOT a form of active transport?
Na+/K+ pump
Glucose Transporter/uniporter
Na+/Glc Symport
ABC ATPase
None of the above
Which small ion transport mechanism is NOT a form of active transport?
Na+/K+ pump
Glucose Transporter/uniporter
Na+/Glc Symport
ABC ATPase
None of the above
Vesicle fusion step involves
Binding of coat proteins to v-SNAREs
Dynamin-facilitated membrane mixing at the fusion site
Insertion of water molecules into the phospholipid bilayers of vesicles and target organelle.
None of the above
Vesicle fusion step involves
Binding of coat proteins to v-SNAREs
Dynamin-facilitated membrane mixing at the fusion site
Insertion of water molecules into the phospholipid bilayers of vesicles and target organelle.
None of the above
What is the first event?
A. Signal sequence is removed once the translocation event has been completed, but before protein translation is completed.
B. Emergence of a hydrophobic signal sequence close to N-terminus of a protein as it is being synthesized in the cytoplasm by a ribosome.
C. The complex of emerging protein, ribosome translating it, mRNA that is being translated, and SRP bind the SRP receptor on ER membrane by direct interaction of SRP and SRP receptor.
D. SRP binds the signal sequence of growing protein and undergoes a conformational change to also bind to the ribosome and pause translation.
What is the first event?
A. Signal sequence is removed once the translocation event has been completed, but before protein translation is completed.
B. Emergence of a hydrophobic signal sequence close to N-terminus of a protein as it is being synthesized in the cytoplasm by a ribosome.
C. The complex of emerging protein, ribosome translating it, mRNA that is being translated, and SRP bind the SRP receptor on ER membrane by direct interaction of SRP and SRP receptor.
D. SRP binds the signal sequence of growing protein and undergoes a conformational change to also bind to the ribosome and pause translation.
Monomeric G-proteins are never free of Guanine nucleotides and are either bound to GTP or they are bound to GDP
True
False
Monomeric G-proteins are never free of Guanine nucleotides and are either bound to GTP or they are bound to GDP
True
False
How is channel-mediated transport different from uniport/transporter-mediated transport?
Channel-mediated is order of magnitude faster than transporter-mediated.
Channel mediated involves binding and conformational change but transporter-mediated does not.
Channel mediated is passive transport but transporter mediated is not.
Channel mediated transports small molecules like ions, transporter mediated transports macromolecules like
proteins.
None of the above.
How is channel-mediated transport different from uniport/transporter-mediated transport?
Channel-mediated is order of magnitude faster than transporter-mediated.
Channel mediated involves binding and conformational change but transporter-mediated does not.
Channel mediated is passive transport but transporter mediated is not.
Channel mediated transports small molecules like ions, transporter mediated transports macromolecules like
proteins.
None of the above.
Which of the following is true for The Signal Sequence.
Is a short stretch of positively charged residues.
Is a permanent part of the protein and is not removed after transport.
Interacts with one domain of the ribonucleoprotein Signal Recognition Particle (SRP)
Both A & B
Which of the following is true for The Signal Sequence.
Is a short stretch of positively charged residues.
Is a permanent part of the protein and is not removed after transport.
Interacts with one domain of the ribonucleoprotein Signal Recognition Particle (SRP)
Both A & B
What is the second event?
A. Signal sequence is removed once the translocation event has been completed, but before protein translation is completed.
B. Emergence of a hydrophobic signal sequence close to N-terminus of a protein as it is being synthesized in the cytoplasm by a ribosome.
C. The complex of emerging protein, ribosome translating it, mRNA that is being translated, and SRP bind the SRP receptor on ER membrane by direct interaction of SRP and SRP receptor.
D. SRP binds the signal sequence of growing protein and undergoes a conformational change to also bind to the ribosome and pause translation.
What is the second event?
A. Signal sequence is removed once the translocation event has been completed, but before protein translation is completed.
B. Emergence of a hydrophobic signal sequence close to N-terminus of a protein as it is being synthesized in the cytoplasm by a ribosome.
C. The complex of emerging protein, ribosome translating it, mRNA that is being translated, and SRP bind the SRP receptor on ER membrane by direct interaction of SRP and SRP receptor.
D. SRP binds the signal sequence of growing protein and undergoes a conformational change to also bind to the ribosome and pause translation.
Primary and secondary active transport mechanism allow movement of at least one entity against its concentration gradient
true
false
Primary and secondary active transport mechanism allow movement of at least one entity against its concentration gradient
true
false
The transcellular transport of glucose across the intestinal epithelia cell lining relies on the following for the unidirectional absorption of glucose from the gut.
Low intracellular Na+ concentration maintained by the Na+/K+ pump.
High intracellular potassium (K+) maintained by tight junctions.
High intracellular glucose maintained by glucose transporter at the basal domain/side.
Low intracellular glucose maintained by Na+ driven glucose symport.
None of the above.
The transcellular transport of glucose across the intestinal epithelia cell lining relies on the following for the unidirectional absorption of glucose from the gut.
Low intracellular Na+ concentration maintained by the Na+/K+ pump.
High intracellular potassium (K+) maintained by tight junctions.
High intracellular glucose maintained by glucose transporter at the basal domain/side.
Low intracellular glucose maintained by Na+ driven glucose symport.
None of the above.
Which one of the following occur in both ER and Golgi
Glycosylation
Sorting of secretory proteins from lysosomal enzymes
Removal of the signal sequence
Both B and C
Which one of the following occur in both ER and Golgi
Glycosylation
Sorting of secretory proteins from lysosomal enzymes
Removal of the signal sequence
Both B and C
What is the third event?
A. Signal sequence is removed once the translocation event has been completed, but before protein translation is completed.
B. Emergence of a hydrophobic signal sequence close to N-terminus of a protein as it is being synthesized in the cytoplasm by a ribosome.
C. The complex of emerging protein, ribosome translating it, mRNA that is being translated, and SRP bind the SRP receptor on ER membrane by direct interaction of SRP and SRP receptor.
D. SRP binds the signal sequence of growing protein and undergoes a conformational change to also bind to the ribosome and pause translation.
What is the third event?
A. Signal sequence is removed once the translocation event has been completed, but before protein translation is completed.
B. Emergence of a hydrophobic signal sequence close to N-terminus of a protein as it is being synthesized in the cytoplasm by a ribosome.
C. The complex of emerging protein, ribosome translating it, mRNA that is being translated, and SRP bind the SRP receptor on ER membrane by direct interaction of SRP and SRP receptor.
D. SRP binds the signal sequence of growing protein and undergoes a conformational change to also bind to the ribosome and pause translation.
P-type pumps are the only form of active transport where the pump/transporter is autophosphorylated
True
False
P-type pumps are the only form of active transport where the pump/transporter is autophosphorylated
True
False
What is the last event?
A. Signal sequence is removed once the translocation event has been completed, but before protein translation is completed.
B. Emergence of a hydrophobic signal sequence close to N-terminus of a protein as it is being synthesized in the cytoplasm by a ribosome.
C. The complex of emerging protein, ribosome translating it, mRNA that is being translated, and SRP bind the SRP receptor on ER membrane by direct interaction of SRP and SRP receptor.
D. SRP binds the signal sequence of growing protein and undergoes a conformational change to also bind to the ribosome and pause translation.
What is the last event?
A. Signal sequence is removed once the translocation event has been completed, but before protein translation is completed.
B. Emergence of a hydrophobic signal sequence close to N-terminus of a protein as it is being synthesized in the cytoplasm by a ribosome.
C. The complex of emerging protein, ribosome translating it, mRNA that is being translated, and SRP bind the SRP receptor on ER membrane by direct interaction of SRP and SRP receptor.
D. SRP binds the signal sequence of growing protein and undergoes a conformational change to also bind to the ribosome and pause translation.
Gated ion channels are passive transport mechanisms that open in response to stimuli
True
False
Gated ion channels are passive transport mechanisms that open in response to stimuli
True
False
Coat proteins and dynamic are only involved at budding stage f vesicular trafficking & t-SNARE s/v-SNARES are only involved at fusion step
True
False
Coat proteins and dynamic are only involved at budding stage f vesicular trafficking & t-SNARE s/v-SNARES are only involved at fusion step
True
False