Assays
Reagent used to specifically test for beta-galactosidase.
What is ONPG?
Technique that separates proteins by size.
What is SEC?
Reagent used to precipitate proteins out of solution.
What is ammonium sulphate?
Method we used in lab to break open the cells.
What is sonication?
Type of gels used for protein analysis.
What are polyacrylamide gels?
Universal assay for proteins.
What is the Bradford assay?
Technique used to separate proteins based on charge.
What is IEC?
Method used to separate the precipitated proteins from the supernatant.
What is centrifugation?
Special bath used to maintain a low temperature during sonication.
What is ethanol/ice?
Gels with large pores used specifically for DNA.
What are agarose gels?
Dye used in the Bradford assay.
What is Coomassie blue?
Resin used in IEC to retain negatively charged proteins.
What is DEAE?
Method for desalting proteins that requires a tubular membrane or cassette.
What is dyalisis?
Gentle method for cell disruption that requires adding a reagent.
What is enzyme digestion?
Ionic denaturing reagent added to protein samples before loading on a gel.
What is sodium dodecyl sulfate?
The ratio between enzyme activity and total amount of protein.
What is specific activity?
Resin used in IEC to retain positively charged proteins.
What is CM?
Reagent added to adjust the pH during the salting out phase.
What is sodium hydroxide?
Vigorus method used mainly for breaking plant and bacterial cells.
What is the French Press?
Reagent added to protein samples with the purpose of breaking disulfide bonds.
What is beta-mercaptoethanol?
The chemical responsible for the yellow color in the ONPG assay (full name).
What is ortho-nitro-phenol?
Technique that separate proteins based on their differential affinity for a ligand.
What is affinity chromatography?
Desalting method used industrially for a large volume of protein sample.
What is TFF (Tangential Flow Filtration)?
Break open the during soication.
What are sound waves?
Electrophoresis method that uses a pH gradient gel.
What is isoelectric focusing?