Show:
Catalase & Oxidase
Coagulase
Mannitol Salt
Eosin Methylene Blue Agar
MacConkey Agar
100
What is the purpose of the Catalase test?
To determine if oxygen gas is produced
100
The coagulase test differentiates between what bacteria?
What is Staphylococcus aureus and other gram-positive cocci.
100
Which carbohydrate is used in Mannitol Salt Agar (MSA).
What is mannitol?
100
For the Methylene Blue Agar test does the "P" ID the bacteria as Gram-negative or Gram-positive?
What is Gram-positive
100
What carbohydrate is present in the MacConkey agar?
What is Lactose.
200
What is the purpose of the Oxidase test?
To determine if cytochrome is present
200
Bacteria was added to what type of plasma to perform this test?
What is rabbit plasma.
200
What organism ferments mannitol?
What is Staphylococcus aureus.
200
What is the purpose of the Eosin Methylene Blue Agar test?
What is to determine if the organism is a fecal coliform.
200
Color produced on agar from lactose fermentation at a low pH.
What is red.
300
If bubbles appear after one minute of completing the catalase test, what is this called?
What is a false positive
300
A positive result in the coagulase test indicates?
What is the bacteria is able to produce the coagulase enzyme.
300
Color produced on agar from mannitol fermentation above a pH of 8.4.
What is pink?
300
What is the interpretation of the EMB if the growth color is Pi?
What is Lactose fermentation.
300
Agar formulations lacking this allow growth of Enterococcus and some species of Staphylococcus.
What is crystal violet.
400
What does catalase hydrolyze?
What is hydrogen peroxide into water and oxygen gas
400
Name the two forms of the coagulase test:
What is: 1. Tube test; tests for bound & free coagulase 2. Slide test; tests for bound coagulase only
400
If an organism is pathogenic, what color should the growth color appear to be?
Yellow
400
The organism ferments lactose and/or sucrose and there is a lot of acid production. What is the result and presumptive ID?
What is "D" (Growth is dark with or without a green metallic sheen); and it is a probable coliform.
400
pH indicator that is colorless above a pH of 6.8 and red at a pH below 6.8.
What is Neutral red dye.
500
What is a positive result for the Oxidase test?
What is a change in color (blue)
500
Coagulase works with plasma to form _________ defending themselves from phagocytosis.
What is protective fibrin barriers.
500
The Mannitol Salt Agar is selective for ___________. Most other bacteria cannot survive the high salt levels.
What is staphylococci.
500
What does it mean if an organism does not ferment lactose or sucrose and there is no Reaction.
What is a noncoliform. Growth is colorless (C)
500
Components in Agar that inhibits the growth of Gram-positive bacteria.
What is bile salts and crystal violet.