RFLP Basics
What does RFLP stand for?
restriction fragment length polymorphism
What equipment do we use to separate DNA fragments by their size while analyzing an RFLP
Gel electrophoresis
What type of helix is DNA
A double Helix
How is a RFLP used?
It is used to analyze the length of DNA
Why is it important to use a DNA ladder during gel electrophoresis when analyzing an RFLP sample
To "measure" or esitmate the size of the DNA fragments in a Sample
Which base pairs bind together?
A binds with T, C binds with G.
How is an RFLP measured ?
It is measured in base pairs
Why is it important to run a negative control sample when performing an RFLP
A negative control does not contain a DNA sample and ensures that the other samples have not been contaminated
Name one of the four base pairs
Adenine, thymine, cytosine, and guanine.
What is the visual pattern of a DNA fragment called after performing a gel electrophoresis and staining lab?
DNA Fingerprint
Why was it important to add Chelex to your cells before extreme heat is used to break open the cells?
It protects the DNA from degradation
what is the process of [DNA -> RNA -> protein] called?
Central Dogma
What are the main steps of RFLP?
DNA extraction. DNA digestion with restriction enzymes. Gel electrophoresis. Analyzation.
Using the image, how many BamHI cuts were made and what is the total bp of lane two?
2 BamH1, 400 bp
A segment of DNA containing instructions for a specific protein
A Gene