Microscopy
Give me two examples of characteristics of bacteria under a microscope.
shape, size, arrangement, motility, flagella, spores, capsule.
It divides bacteria into Gram positive and Gram negative
what is gram staining
Clotting of milk, peptonisation or saponification may occur in this test.
Litmus milk
The test should not be considered negative till after four days of incubation.
Urease test
This shape of bacteria is spherical and often forms clusters, like Staphylococcus aureus.
👉 What is cocci?
Two types of arrangements of bacteria.
Any given correct answer
Acid-fast and non- acid fast bacteries can be differentiated by this staining.
Zielh-Neelsen
This test depends on the production of acetyl methyl carbinol from pyruvic acid as an intermediate stage in its conversion to 2:3 butylene glycol.
Voges-Proskauer test
Complete decolourisation is strongly positive,while green is weakly positive in this test.
Methlene blue reduction
This rigid layer made of peptidoglycan gives bacterial cells their shape and protection.
👉 What is the cell wall?
Motility is seen best by ....... ...... method in unstained preparations
What is hanging drop method?
Surface antigens are identified by this technique.
Fluorescent antibody technique
Indole production test demonstrates the production of indole from this substance.
Tryptophane.
The test reagent consists of a mixture of equal volumes of solutions of sulphanilic acid and a-naphthylamine in 5N acetic acid mixed just before use. 0.1 ml of the test reagent is added to the culture. What kind of change in this test we can see if the test is positive?
red colour
Bacteria that can survive without oxygen but grow better with it are called this type.
👉 What are facultative anaerobes?
Vibrios have 3 arrangements:
single, in S, spiral forms.
Gullola, show figure 1 and they should name the staining used.
Ziehl Neelsen
This test is used to detect the production of acid during the fermentation of glucose and the maintenance of pH below 4.5 in an old culture.
Methly red test
This organism produces lecithnase, when grown on a solid egg yolk medium form colonies surrounded by a zone of clearing.
C. perfringens
This bacterial structure allows pathogens like Neisseria gonorrhoeae to attach firmly to host cells.
👉 What are pili (fimbriae)?
Count the types of bacteria according to flagella existence.
Mainly, Ziehl Neelsen is used for identifying ..... bacteria
What is Mycobacterium tuberculosis
These tests are very useful in the identification and classification of enteric Gram negative bacteria.
IMViC
The test can also be done by Kovac's method.
Oxidase reaction
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