General DNA
Replication Proteins
History
PTC
PCR
100
Identify the parts of a Nucleotide from right to left:
Nitrogenous Base
Deoxyribose Sugar
Phosphate Group
100
Which protein is responsible for extending the synthesized strand of DNA?
DNA Polymerase
100
Who was responsible for identifying the general structure of DNA (2 people)
Watson & Crick
100
Is the bitter tasting gene dominant or recessive?
Dominant
100
What does PCR stand for?
Polymerase Chain Reaction
200
In which direction is DNA synthesized?
5' --> 3'
200
What protein adds RNA primers to a template strand, marking the replication site for DNA polymerase?
DNA Primase
200
Who used X-ray Crystallography to visualize DNA?
Rosalind Franklin
200
What would be the evolutionary need for a gene to taste PTC?
Survival, tasting bitter substances could mean they are toxic
200
Assuming we started with 1 strand of DNA, how much DNA would we have after 13 PCR cycles?
8,192
300
When replicating DNA, both strands are kept intact and separated so that each strand can be used as a template for the synthesized complimentary strand. What is this an example of?
Semiconservative Replication
300
On what template strand of DNA can Okazaki fragments be found?
Lagging Strand
300
Who proved that DNA could be replicated invitro with the assistance of DNA Polymerase?
Arthur Kornberg
300
Which allele (Dominant or Recessive) can be cut with the HaeIII Restriction Enzyme?
Dominant
300
From what organism was Taq Polymerase isolated?
Thermus Aquaticus
400
Separate the Nucleotide bases into Purines and Pyrimidines
Purines: Adenine, Guanine
Pyrimidines: Cytosine, Thymine, Uracil
400
Which protein prevents the separated strands of DNA from binding back together?
Single Stranded Binding Proteins (SSBs)
400
Who invented the PCR Technique?
Kary Mullis
400
Assume wells 2-6 hold student digested samples. What is the genotype for each?
2: tt
3: Tt
4: TT
5: tt
6: Tt
400
What makes dNTPs different from traditional Nucleotides?
3 phosphate groups instead of 1
500
Define Anti-parallel in relation to DNA
Parallel, but going in opposite directions
500
Which protein helps unwind the DNA, prepping it for separation?
Topoisomerase
500
Organize the following scientists from most recent work to oldest work:
Arthur Kornberg
Kary Mullis
Rosalind Franklin
Watson & Crick
Kary Mullis
Arthur Kornberg
Watson & Crick
Rosalind Franklin
500
When looking at an electrophoresis gel, if we see the expected results in our undigested sample and notice 5 bands in our digested sample, what would have occurred?
Likely contamination of additional restriction enzymes (possibly from skin/hair/breath/air)
500
Why do we need both a forward and reverse primer?
To flank the Gene of Interest (GOI), and so that the primers don't bind to each other in the reaction.