64
Melee
Brawl
4
Ultimate
100

The main antagonist.

Who is Master Hand?

100

This was Mario's first appearance in a game with an ESRB rating higher than E, at this rating. 

What is T (for Teen)?

100
This character makes his international debut in Brawl.

Who is Lucas?

100

The only game in the series where this character is playable from the start.

Who is Luigi?

100

This character was finally playable in the series in this game, being a long time request and appearing in Brawl and 4 as non-playable characters. The reason was referenced in the announcement trailer for the character.

Who is Ridley?

200

This character was replaced by Jigglypuff.

Who is Pit?

200

In the glitch where you can play as Master Hand, only the player of this color can control him.

What is yellow?

200
This character was voiced by Masahiro Sakurai.

Who is King Dedede?

200

This stage is a Battlefield reskin, or at least very similar.

What is Miiverse?

200

The only character to always be a starting character.

Who is Kirby?

300

The only N64 game to feature these two characters.

Who are Samus Aran and Ness?

300

This event match references a GameCube tech demo.

What is Super Mario 128?

300

This is the first game in the series where this character has a unique voice, albeit still by the same voice actor.

Who is Luigi?

300
This version allows the use of the other version as a controller.

What is For Wii U?

300

These three voice actors were credited posthumously for their work, which appeared as reused voice clips.

Who are Unshō Ishizuka, Takeshi Aono, and Kaneto Shiozawa?

400

The only playable character that has been a villain.

Who is Donkey Kong?

400

This series had no game in North America before Melee came out.

What is Fire Emblem?

400

This person also voiced the narrator in Bill Nye the Science Guy.

Who is Pat Cashman?

400

This version is the reason the Ice Climbers were scrapped.

What is For 3DS?

400

These characters had memes referenced by Nintendo Versus or Masahiro Sakurai himself.

Who are Piranha Plant, Yoshi, and Mario?

500

The idea of characters entering on floating platforms came from this game.

What is Mario Bros?

500

These two characters were requested by their original developers, but were left out due to time constraints.

Who are Sonic the Hedgehog and Solid Snake?

500

These 3 characters did not show up in SubSpace Emissary until after you beat the game.

Who are Toon Link, Jigglypuff, and Wolf?

500

These two moves are the only custom special moves to have Kirby copy voice lines.

What are Heavenly Light and Explosive Flame?

500

The first game in the series to use these terms, techniques, and name.

What are tilt, neutral, edge guarding, dash dancing, and Pneuma?