FAUNA
BACTERIA/ARCHAEA
FUNGI
MOLECULAR METHODS
LABS
100
rotifer
100
in what way do bacteria reproduce differently than archaea?
spores
100
main cellular structure difference between fungi and bacteria?
chitin in fungus
100
what kind of bases are these?
pyrimidine
100
describe the objective for the algae MPN lab
enumerate algae using the most probable number technique
200
What controls trophic webs from the bottom up?
Resource limitation
200
wayne-gretzky column describes
redox gradient from aerobic to anaerobic conditions
200
What are the fungi structures?
thallus
mycelium
hyphae
200
how can marker genes be used to study plant/microbe interactions
clones into cell (chromosomes in fungi/ plasmids in bacteria)
200
soil moisture correction formula
g dry soil = g moist soil / (1 + θ g)
300
What controls trophic levels within the food web from the top down?
predation, mutualism, facilitation
300
Photoheterotrophs vs chemoautotrophs energy and carbon source
photoautotrophs: CO2 for carbon, light for energy
chemoautotrophs: CO2 carbon, inorganic compounds for energy
300
Which class of fungus is this?
Ascomycota
300
What are the necessary components to perform PCR?
DNA primers, TAQ polymerase, spare nucleotides,
300
objective for stinking mud lab?
demonstrate biogeochemical and redox processes involved in the formation of redoximorphic features as a result of microbial respiration
400
micro-, meso-, and macro fauna sizes
1 - 100 microns
100 microns - 2 mm
2mm - meter scale
400
how do bacteria transfer information?
conjugation
transduction
translation
400
describe at least 3 functions
organic matter production
decomposition
N and P cycling
mycorrhizal associations for water/nutrients
resistance to thermal and drought stress
reduce herbivory
support C and N fixation (lichen)
stabilize soil surface (biotic crusts)
carbon sequestration
400
describe the steps to performing PCR?
Denaturing
annealing
synthesizing
repeat
400
given the table of values, how would you find the algae MPN?
divide number of the three representative test tubes set you choose by the weight of dry soil
500
Name different protozoans
how are they different?
flagellates, naked amoebas, testate amoebas, ciliates
500
Difference in cell walls?
Archaea: pseudomurein, heteropolysaccharides
Bacteria: peptidoglycan
500
explain one of the 5 methods of analyzing nucleic acid extracts
DNA:DNA reassociation kinetics
microarrays
Restriction Fragment length polymorphism analysis
cloning and sequencing
stable isotope probing