Tubes
Lavender
what is the correct order of draw?
Gold, Lavender , Green, Light Blue , Blood Cultures, Pink , Grey, Royal Blue
Blood Cultures, Light Blue , Gold, Green, Lavender, Pink, Grey, Royal Blue
Why is it important to collect a blood culture sample using strict aseptic technique?
Blood cultures are used to detect infections in the bloodstream. Contamination with skin flora due to improper technique can lead to false-positive results, which may result in unnecessary antibiotic treatment or misdiagnosis.
what do you need to do when drawing a bilirubin
protect from light
the antiseptic most commonly used to prepare for venipuncture is a 70%
isopropyl alcohol
complete blood counts(CBC) must be done on what type of blood
Whole blood
Whats the order of draw ( additives)
Heparin, Sodium Citrate , EDTA
Sodium Citrate, Heparin, EDTA
What is the proper handling and storage requirement for a 24-hour urine collection, and what is a common reason for rejection of the specimen?
The specimen should be kept refrigerated or on ice throughout the collection period. A common reason for rejection is missing a void during the collection period, which makes the sample incomplete and invalid for accurate analysis.
24-Hour Urine Collection
Keep the collection container refrigerated or on ice.
• Avoid certain foods, drinks, or medications if instructed.
if a family member or nurse helps to confirm the identity of a nonverbal or unresponsive patient, the PBT must document what information of the individual who helps
name
where can you find the labels for additives and expiration dates
On the tube
whats the order of draw?
Royal Blue,light blue, green,pink
light Blue, green, Pink, Royal blue
What is the required patient preparation for a fasting glucose test, and why is it necessary?
The patient must fast for 8-“12 hours, consuming only water. This ensures that recent food intake does not artificially elevate glucose levels
Fasting Blood Test
Patient must fast for 8–12 hours before the test.
Only water is allowed; no food, coffee, or sugary drinks.
Avoid alcohol and heavy exercise before the test.
what must be done with a phlebotomy wedge after each use
must be sanitized
ACD is found in what tube
Yellow
whats the right order of draw
gold,Green,gray,Royal Blue, light Blue,
gold, light Blue, green,gray, Royal blue
Why is a first-morning sputum sample preferred for AFB (Acid-Fast Bacillus) testing, and how should the patient collect it properly?
 A first-morning sample is preferred because secretions accumulate overnight, making the bacterial load higher, increasing the likelihood of detecting Mycobacterium tuberculosis. The patient should rinse their mouth with water, take deep breaths, and cough deeply from the lungs (not the throat) into a sterile container to ensure a proper specimen.
What is the term for errors that happen before a specimen is tested?
Pre-analytical errors
how are blood draws usually done on young pediatric patients in a general setting
with a parent or guardian holding the child
what additive does a royal blue tube contain
EDTA
whats the order of draw
potassium oxalate, lithium heparin,Sodium polyanethol sulfonate
lithium heparin(green), potassium oxalate (gray), polyanethol sulfonate (yellow)
A patient is scheduled for a timed vancomycin trough level test. When should the blood sample be drawn, and why?
The blood should be drawn just before the next dose of vancomycin is administered. This ensures that the lowest concentration (trough level) of the drug in the bloodstream is measured, helping to determine if dosing is effective and preventing toxicity.
Timed Hormone/ Medication levels
Blood must be drawn at a specific time
antiseptics containing iodine should not be used on patients who have an allergy to
shellfish