Barcoding 101
This is the targeted region of Mitochondrial DNA used for barcoding invertebrates/insects.
What is COI?
This process has bacteria take up foreign DNA, generally in the form of plasmids, and provides the bacteria with new characteristics.
- Elizabeth
What is bacterial transformation?
In the DNA molecule, this is the type of chemical bond that holds nitrogenous bases together as pairs.
What is a Hydrogen Bond?
This kingdom of life contains all insects, fish, and mammals.
What is Animalia / Animals?
This is Katrina's last name.
Spelling Counts!
What is Giambertone?
This is the targeted region of Chloroplast DNA commonly used to barcode plant samples.
What is RBCL?
These are the two types of chemical transformations.
-Emma
What are...
1. Rapid Transformation
2. Competent (classic) Transformation
This is the function of Endonucleases.
- Nicole
What is an enzyme that cuts DNA at a specific sequence within the molecule?
This is the kingdom of life that contains all bacteria.
What is Monera?
This is Kelly's last name.
Spelling Counts!
What is Eames?
This is the targeted region commonly used to barcode bacteria samples.
What is 16S?
This is the phase of growth that most bacteria are at when going through a Rapid Transformation.
- Emily
What is the stationary phase?
When running a gel, these sized fragments of DNA move the furthest from the wells.
What are the smallest sized fragments?
This is the name of the type of tree like image commonly used to show relationships between species over an evolutionary time scale.
What is a Phylogeny / Phylogenetic Tree?
This is Jeff's last name.
Spelling Counts!
What is Petracca?
This buffer solution is used to break open the cells and release the DNA during a Silica Isolation.
(This was recommended by one of you... don't blame me)
What is Lysis Buffer?
Competent Transformations are approximately this number of times more efficient than a Rapid Transformation.
What is 4 - 6 times more efficient?
When running a gel, this is one of the reasons you may not see any results in a lane.
(There are at least 4 possible answers - answer with 4 and receive double points)
What is...
1. Stabbed the well when loading
2. Missed the well when loading
3. Mis-measured when setting up the reaction and no DNA was in the tube
4. Accidentally added Chelex to the PCR tube and prevented amplification of the target region
The order Coleoptera contains all species of this type of organism (needs to be more specific than insects).
What are Beetles?
This is the name of the mummy replica in the museum at the DNA Learning Center.
What is Otzi the Iceman?
This process works by comparing the genetic sequence of an unknown sample to a reference database of known sequences.
What is DNA Barcoding?
Kanamycin and Ampicillin are two antibiotics that work in different ways to kill bacteria. This is how they do it.
What is...
1. Kanamycin prevents protein synthesis
2. Ampicillin prevents cell division
DOUBLE JEOPARDY! FULL ANSWERS RECEIVE DOUBLE POINTS. INCOMPLETE RECEIVES 0.
This is what the lane of a gel would look like when running the following experiment:
A double transformation with the pKAN and Simple Recombinant plasmids, digested by BamHI/HinDIII.
What is.... whatever Kelly is drawing?
(The free version doesn't let me add images. Sorry not sorry)
This is the name of the person who formalized the modern system used for naming and classifying organisms.
Who is Carl Linneaus?
This is the name of your favorite DNA Learning Center instructor.
What is.... A TRAP THAT YOU FELL FOR HAHAHA