Nucleic Acids
DNA Replication
Proteins
Mutations
Separation Techniques
100
Name all of the nitrogenous bases in RNA.
What is adenine, guanine, cytosine, and uracil.
100
Which enzyme unzips the DNA?
What is helicase.
100
What amino acid does the codon AUG code for.
What is methionine.
100
List the three effects a mutation can have on an organism?
What is detrimental effect, neutral effect, and beneficial effect.
100
What is the purpose of PCR?
What is to amplify millions of copies of a specific sequence of DNA.
200
What type of bond makes up the sugar-phosphate backbone?
What are covalent bonds?
200
What is the function of the enzyme topoisomerase?
What is to relieve supercoiling.
200
What are the four levels of protein structure?
What are primary, secondary, tertiary, and quaternary.
200
What type of mutation affects an entire organism and can be passed down to their offspring?
What is a germ-line mutation.
200
What is the name of the polymerase used in PCR?
What is Taq polymerase.
300
Which nitrogenous bases are purines?
What is adenine and guanine?
300
Which direction does DNA Polymerase read the template strand?
What is 3' to 5'.
300
What are the three stop codons?
What are UAA, UAG, and UGA.
300
Provide an example of a transversion mutation.
What is a AT to TA or CG to GC.
300
Calculate the Rf of analyte A.
What is 0.75 (3/4).
400
How many carbon ring(s) do pyrimidines have?
What is one carbon ring.
400
Which direction is the DNA on the lagging strand synthesized?
What is 3' to 5'.
400
During posttranscriptional modification, which segments of RNA are removed?
What are introns.
400
Which type of mutation is most severe?
What is a frameshift mutation.
400
What do all of the letters stand for in the term SDS-PAGE?
What is sodium dodecyl-sulfate polyacrylamide gel electrophoresis.
500
Which end of DNA has a hydroxyl group?
What is 3' end.
500
What is the function of single stranded binding proteins?
What is to stop the separated DNA strands from joining back together.
500
What two things are added to mRNA before it leaves the nucleus?
What are the poly-A tail and the guanosine cap.
500
What type of mutation causes Sickle Cell Disease?
What is a missense mutation?
500
What is the name of the bacteria that Taq polymerase is made from?
Thermus Aquaticus