Meiosis
This stage of Meiosis I is the period of growth that allows distribution of genetic material
What is Interphase I?
If only DNA had sunscreen to protect from this degradation source
What is UV Radiation?
This profile artifact is typically caused by too much DNA input
What is Pull-up?
This DNA extraction reagent is comprised of toxic and carcinogenic chemicals. No wonder it is no longer used at most forensic labs anymore!
What is Phenol-Chloroform?
This forensically relevant enzyme helps to break down carbohydrates and starches
What is Amylase?
This stage of Meiosis II ends with 4 haploid daughter cells created
What is Telophase II/Cytokinesis II?
Humic acid is a component of this material known to degrade DNA
What is Soil?
This phenomenon during DNA replication is frequently associated with causing stutter peaks during capillary electrophoresis
What is Strand Slippage?
This medical test was once used as a forensic identification method despite its minimally discriminatory nature
What is ABO Typing?
This enzyme is coveted in forensic circles for its thermal resistance at high temperatures
What is Taq Polymerase?
What is Metaphase I?
This common household cleaner is an effective degradation agent for DNA. Just don't mix it with ammonia!
What is Bleach?
This profile artifact is caused by reflective material within the POP-4 polymer or any substance that comes across the CCD camera
What is a Spike?
This method for genetic sequencing utilizes VNTR's and restriction sites
What is Restriction Fragment Length Polymorphism (RFLP)?
This enzyme is responsible for connecting Okazaki Fragments to create one continuous strand
What is Ligase?
In this stage of Meiosis I, chromosomes are pulled towards opposite poles
What is Anaphase I?
These two degradation agents prove that not even DNA can survive Florida!
What are Humidity and Heat?
Tri-allelic peak patterns are abnormal and could be caused by this "mythical" medical phenomenon
What is Genetic Chimerism?
This forensic DNA typing method utilizes a polymorphic gene found in the leukocyte antigen system
What is HLA-DQa analysis?
This enzyme breaks down RNA primers towards the end of the DNA replication process. However, it can also degrade DNA if left to its own devices!
What is Nuclease?
In this stage of Meiosis II, spindles form and nuclei disintegrate in preparation for cell division
What is Prophase II?
DNA is fragile, particularly after amplification. Be careful as this major step in reagent preparation can actually degrade DNA!
What is Vortexing?
Allelic dropout is frequently associated with degradation and this lesser considered factor. Think DNA replication!
What is a Primer Binding Site Mutation?
This traditional DNA quantification procedure utilizes the southern blotting method
What is Slot Blot?
This enzyme helps to stabilize the structure of DNA as it gets unwound and lysed by helicase
What is Topoisomerase?