Upstream
How many Flask do we use for thaw?
2
What is the DF buffer in UF3
WFI
What is the most expensive reagent added to the IVT SUM
Clean Cap
What does DV stand for?
Diavolume
What is Ahmed favorite Car
Mercades
What is the type of centrifuge we use?
Disk Stack
How many Pre-treatments of Resin do we need?
3
How many dia-volumes are required at DF1 and DF2?
5 and 10
What does NPCW stand for
Non potable cold water
Name the ID of the BSC in Ferm Sample Room
BSC2135
How many fixed assets (tanks, Etc) are in Fermentation area
21
What is generated during chromatography that is used to trend product collection/impurities
Chromatogram
What is the total membrane area for the membranes at the 112L scale
21.0m^2
What does the 260 in A260 mean
The wavelegnth being measured in 260nm
What is the highest mixing speed once allowed in Suite J
180RPM
Name the Buffer Tanks for Lysis and Materials
TK3442 - Flocc Buffer
TK3444 - Neutralization
TK3445 - Lysis
TK3441 - Resuspension
What is the volume of HEPEs addition during UF3 and the required concentration
10%, >0.8 g/L
What is the target grams of mRNA to add to each FFT bag during fill
20.1
Name all the major assets located inside purification:
CPS3246, CC1216, CC1217, UF1063, TK3439, TK3461, TK3438, TK3439, TK3457
Why is the pressure differential between Fermentation and Fermentation passthrough Significant?
Because pressure travels from high to low. We don’t want air to travel from the passthrough to the suite, we want the other way around This is to maximize cleanliness of the air and reduce any potential contaminants.
The Sugar Question:
What is the concentration of Dextrose ?
and
What is the length of fermentation post Glucose continuous feed?
702 g/L
31 +/- 2 hrs
What is the Full volume of the Column at 61.3CM
481L
How large is the mRNA (unit=kDA)
350 kDA
Name all buffers we use in suite J (Items that start with an H, no DNA)
H000023929 storage, H000023930 sani, H000023931 (10x), H000023952 (cacl2), H000023953 Pre UF dilution, H000023954 DF1, H000023955 DF2, H000030772 (IVT EDTA), H000031220 mag acetate
What is the total area of Suite J (Rounded to the nearest thousand) in Ft^2?
2000ft^2 (2132ft^2)